Skip to main content
Journal cover image

Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures.

Publication ,  Journal Article
Olazagasti, JM; Hein, M; Crowson, CS; de Padilla, CL; Peterson, E; Baechler, EC; Reed, AM
Published in: J Inflamm (Lond)
2015

OBJECTIVE: Recently adipokines have been implicated in the regulation of immune and inflammatory responses in autoimmune disease. To investigate the role of adipokines in adult and pediatric patients with newly diagnosed dermatomyositis (DM), we analyzed peripheral blood and skeletal muscle gene expression of four adipokines: visfatin, leptin, adiponectin and resistin. METHODS: Peripheral blood mononuclear cells (PBMCs) were collected for 21 adult DM, 26 juvenile DM, 5 non-disease adult controls, and 6 non-disease pediatric controls at two time points: baseline and 6 months. Muscle biopsies from 5 adult DM patients and 5 non-disease adult controls were collected at baseline. Similarly, muscle biopsies from 7 juvenile DM patients and 5 non-disease pediatric controls were collected at baseline. The gene expression levels of leptin, adiponectin, resistin, visfatin and related inflammatory cytokines, IL-6, TNF- α, and housekeeping genes GAPDH, B2M, and ACTB were generated using a custom RT(2) Profiler PCR Array. RESULTS: Visfatin gene expression levels in peripheral blood were significantly higher in newly diagnosed adult DM cases compared to non-disease controls (P = 0.004) and these levels correlated with baseline clinical parameters such as age (r = 0.34, P = 0.020), male sex (r = -0.35, P = 0.017), prednisone use (r = -0.42, P = 0.006), and DMARD use (r = 0.35, P = 0.025). No significant association was found between change in visfatin gene expression levels and change in disease activity measures. While visfatin gene expression was significantly up-regulated in muscle tissue of juvenile DM patients (P = 0.028), in adult DM patients only a trend towards significance was observed (P = 0.08). Also, muscle gene expression levels of resistin were significantly elevated in both adult and juvenile DM patients compared respectively to non-disease adult and pediatric controls. Furthermore, an association between peripheral blood resistin gene expression and DM disease activity, including global, muscle, and extra-skeletal disease activity was also observed. CONCLUSION: Peripheral blood visfatin gene expression and muscle resistin gene expression are significantly increased in newly diagnosed adult DM patients. Further longitudinal studies should explore the possibility of using gene expression levels of adipokines such as visfatin and resistin as novel clinical diagnostic biomarkers in DM.

Duke Scholars

Published In

J Inflamm (Lond)

DOI

ISSN

1476-9255

Publication Date

2015

Volume

12

Start / End Page

29

Location

England

Related Subject Headings

  • Immunology
  • 3202 Clinical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 1103 Clinical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Olazagasti, J. M., Hein, M., Crowson, C. S., de Padilla, C. L., Peterson, E., Baechler, E. C., & Reed, A. M. (2015). Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures. J Inflamm (Lond), 12, 29. https://doi.org/10.1186/s12950-015-0075-2
Olazagasti, Jeannette M., Molly Hein, Cynthia S. Crowson, Consuelo Lopez de Padilla, Erik Peterson, Emily C. Baechler, and Ann M. Reed. “Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures.J Inflamm (Lond) 12 (2015): 29. https://doi.org/10.1186/s12950-015-0075-2.
Olazagasti JM, Hein M, Crowson CS, de Padilla CL, Peterson E, Baechler EC, et al. Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures. J Inflamm (Lond). 2015;12:29.
Olazagasti, Jeannette M., et al. “Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures.J Inflamm (Lond), vol. 12, 2015, p. 29. Pubmed, doi:10.1186/s12950-015-0075-2.
Olazagasti JM, Hein M, Crowson CS, de Padilla CL, Peterson E, Baechler EC, Reed AM. Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures. J Inflamm (Lond). 2015;12:29.
Journal cover image

Published In

J Inflamm (Lond)

DOI

ISSN

1476-9255

Publication Date

2015

Volume

12

Start / End Page

29

Location

England

Related Subject Headings

  • Immunology
  • 3202 Clinical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 1103 Clinical Sciences