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Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein.

Publication ,  Journal Article
Anderson, S; Srajer, V; Pahl, R; Rajagopal, S; Schotte, F; Anfinrud, P; Wulff, M; Moffat, K
Published in: Structure
June 2004

We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen bond network to the chromophore. Although structural change occurs within a few nanoseconds on and around the chromophore, it takes milliseconds for a distinct pattern of tertiary structural change to fully progress through the entire molecule, thus generating the putative signaling state. Remarkably, the coupling between the chromophore conformation and the tertiary structure of this small protein is not tight: there are leads and lags between changes in the conformation of the chromophore and the protein tertiary structure.

Duke Scholars

Published In

Structure

DOI

ISSN

0969-2126

Publication Date

June 2004

Volume

12

Issue

6

Start / End Page

1039 / 1045

Location

United States

Related Subject Headings

  • Time Factors
  • Temperature
  • Spectrophotometry
  • Proteins
  • Protein Structure, Tertiary
  • Protein Conformation
  • Protein Binding
  • Photoreceptors, Microbial
  • Mutation
  • Models, Molecular
 

Citation

APA
Chicago
ICMJE
MLA
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Anderson, S., Srajer, V., Pahl, R., Rajagopal, S., Schotte, F., Anfinrud, P., … Moffat, K. (2004). Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. Structure, 12(6), 1039–1045. https://doi.org/10.1016/j.str.2004.04.008
Anderson, Spencer, Vukica Srajer, Reinhard Pahl, Sudarshan Rajagopal, Friedrich Schotte, Philip Anfinrud, Michael Wulff, and Keith Moffat. “Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein.Structure 12, no. 6 (June 2004): 1039–45. https://doi.org/10.1016/j.str.2004.04.008.
Anderson S, Srajer V, Pahl R, Rajagopal S, Schotte F, Anfinrud P, et al. Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. Structure. 2004 Jun;12(6):1039–45.
Anderson, Spencer, et al. “Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein.Structure, vol. 12, no. 6, June 2004, pp. 1039–45. Pubmed, doi:10.1016/j.str.2004.04.008.
Anderson S, Srajer V, Pahl R, Rajagopal S, Schotte F, Anfinrud P, Wulff M, Moffat K. Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. Structure. 2004 Jun;12(6):1039–1045.
Journal cover image

Published In

Structure

DOI

ISSN

0969-2126

Publication Date

June 2004

Volume

12

Issue

6

Start / End Page

1039 / 1045

Location

United States

Related Subject Headings

  • Time Factors
  • Temperature
  • Spectrophotometry
  • Proteins
  • Protein Structure, Tertiary
  • Protein Conformation
  • Protein Binding
  • Photoreceptors, Microbial
  • Mutation
  • Models, Molecular