Skip to main content

Dynamics of Circulating γδ T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma.

Publication ,  Journal Article
Beck, BH; Kim, H; O'Brien, R; Jadus, MR; Gillespie, GY; Cloud, GA; Hoa, NT; Langford, CP; Lopez, RD; Harkins, LE; Lamb, LS
Published in: PLoS One
2015

Human γδ T cells are potent effectors against glioma cell lines in vitro and in human/mouse xenograft models of glioblastoma, however, this effect has not been investigated in an immunocompetent mouse model. In this report, we established GL261 intracranial gliomas in syngeneic WT C57BL/6 mice and measured circulating γδ T cell count, phenotype, Vγ/Vδ repertoire, tumor histopathology, NKG2D ligands expression, and T cell invasion at day 10-12 post-injection and at end stage. Circulating γδ T cells transiently increased and upregulated Annexin V expression at post-tumor day 10-12 followed by a dramatic decline in γδ T cell count at end stage. T cell receptor repertoire showed no changes in Vγ1, Vγ4, Vγ7 or Vδ1 subsets from controls at post-tumor day 10-12 or at end stage except for an end-stage increase in the Vδ4 population. Approximately 12% of γδ T cells produced IFN-γ. IL-17 and IL-4 producing γδ T cells were not detected. Tumor progression was the same in TCRδ-/- C57BL/6 mice as that observed in WT mice, suggesting that γδ T cells exerted neither a regulatory nor a sustainable cytotoxic effect on the tumor. WT mice that received an intracranial injection of γδ T cells 15m following tumor placement showed evidence of local tumor growth inhibition but this was insufficient to confer a survival advantage over untreated controls. Taken together, our findings suggest that an early nonspecific proliferation of γδ T cells followed by their depletion occurs in mice implanted with syngeneic GL261 gliomas. The mechanism by which γδ T cell expansion occurs remains a subject for further investigation of the mechanisms responsible for this immune response in the setting of high-grade glioma.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

5

Start / End Page

e0122387

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • Receptors, Antigen, T-Cell, gamma-delta
  • Mice, Inbred C57BL
  • Interleukin-4
  • Interleukin-17
  • Humans
  • Glioma
  • General Science & Technology
  • Disease Models, Animal
  • Cell Line, Tumor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Beck, B. H., Kim, H., O’Brien, R., Jadus, M. R., Gillespie, G. Y., Cloud, G. A., … Lamb, L. S. (2015). Dynamics of Circulating γδ T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma. PLoS One, 10(5), e0122387. https://doi.org/10.1371/journal.pone.0122387
Beck, Benjamin H., Hyunggoon Kim, Rebecca O’Brien, Martin R. Jadus, G Yancey Gillespie, Gretchen A. Cloud, Neil T. Hoa, et al. “Dynamics of Circulating γδ T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma.PLoS One 10, no. 5 (2015): e0122387. https://doi.org/10.1371/journal.pone.0122387.
Beck BH, Kim H, O’Brien R, Jadus MR, Gillespie GY, Cloud GA, et al. Dynamics of Circulating γδ T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma. PLoS One. 2015;10(5):e0122387.
Beck, Benjamin H., et al. “Dynamics of Circulating γδ T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma.PLoS One, vol. 10, no. 5, 2015, p. e0122387. Pubmed, doi:10.1371/journal.pone.0122387.
Beck BH, Kim H, O’Brien R, Jadus MR, Gillespie GY, Cloud GA, Hoa NT, Langford CP, Lopez RD, Harkins LE, Lamb LS. Dynamics of Circulating γδ T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma. PLoS One. 2015;10(5):e0122387.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

5

Start / End Page

e0122387

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • Receptors, Antigen, T-Cell, gamma-delta
  • Mice, Inbred C57BL
  • Interleukin-4
  • Interleukin-17
  • Humans
  • Glioma
  • General Science & Technology
  • Disease Models, Animal
  • Cell Line, Tumor