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MicroRNA-122 associates with serum apolipoprotein B but not liver fibrosis markers in CHC genotype 1 infection.

Publication ,  Journal Article
Lee, T-H; Matta, B; King, BD; Hodges, MR; Tillmann, HL; Patel, K
Published in: J Med Virol
October 2015

miR-122 is the predominant liver miRNA that regulates hepatic lipid metabolism and inflammation. Hepatitis C virus (HCV) modulates host intracellular lipid metabolism. HCV stability and propagation also depend on an interaction between virus and miR-122. Our aims were to examine the associations between miR-122, apolipoproteins, and serum makers of fibrosis in chronic hepatitis C (CHC) patients. We evaluated baseline sera from 36 CHC genotype 1 patients who completed the Phase IIa study of miravirsen (LNA oligonucleotide targeting miR-122). Samples were assessed for liver transaminases, IL 28B genotype, IP-10, and lipid profiles. The noninvasive markers of liver fibrosis, APRI, and FIB-4, were calculated using standard formulae. miR-122 levels were measured using RT-PCR and expressed as fold-change compared to normal healthy controls. CHC patients were mostly male (61%) with mean age 47.5 ± 11.6 years. Patients with higher ApoB (ApoB/ULN ≥ 0.5) has significantly lower miR-122 levels in compared to patients with lower ApoB (ApoB/ULN < 0.5). (8.28 ± 6.23 vs. 16.28 ± 13.71; P = 0.02). There were no similar associations between miR-122 and ApoA-1 or between HCV RNA and lipoproteins. There were no differences in miR-122 levels between patients with different stages of fibrosis determined by APRI or FIB-4. Patients with lower ApoB had higher serum miR-122 levels. However, we cannot identify significant association between miR-122, ApoA-1, or fibrosis markers in this small cohort of CHC genotype 1 patients. The mechanism of HCV dyslipidemia is complex and could partly relate to the effect of miR-122 on lipid metabolism which requires further evaluation in a larger study.

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Published In

J Med Virol

DOI

EISSN

1096-9071

Publication Date

October 2015

Volume

87

Issue

10

Start / End Page

1722 / 1726

Location

United States

Related Subject Headings

  • Young Adult
  • Virology
  • Severity of Illness Index
  • Real-Time Polymerase Chain Reaction
  • Oligonucleotides
  • Middle Aged
  • MicroRNAs
  • Male
  • Liver Cirrhosis
  • Liver
 

Citation

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Lee, T.-H., Matta, B., King, B. D., Hodges, M. R., Tillmann, H. L., & Patel, K. (2015). MicroRNA-122 associates with serum apolipoprotein B but not liver fibrosis markers in CHC genotype 1 infection. J Med Virol, 87(10), 1722–1726. https://doi.org/10.1002/jmv.24230
Lee, Tzu-Hao, Bassem Matta, Bernard D. King, Michael R. Hodges, Hans L. Tillmann, and Keyur Patel. “MicroRNA-122 associates with serum apolipoprotein B but not liver fibrosis markers in CHC genotype 1 infection.J Med Virol 87, no. 10 (October 2015): 1722–26. https://doi.org/10.1002/jmv.24230.
Lee T-H, Matta B, King BD, Hodges MR, Tillmann HL, Patel K. MicroRNA-122 associates with serum apolipoprotein B but not liver fibrosis markers in CHC genotype 1 infection. J Med Virol. 2015 Oct;87(10):1722–6.
Lee, Tzu-Hao, et al. “MicroRNA-122 associates with serum apolipoprotein B but not liver fibrosis markers in CHC genotype 1 infection.J Med Virol, vol. 87, no. 10, Oct. 2015, pp. 1722–26. Pubmed, doi:10.1002/jmv.24230.
Lee T-H, Matta B, King BD, Hodges MR, Tillmann HL, Patel K. MicroRNA-122 associates with serum apolipoprotein B but not liver fibrosis markers in CHC genotype 1 infection. J Med Virol. 2015 Oct;87(10):1722–1726.
Journal cover image

Published In

J Med Virol

DOI

EISSN

1096-9071

Publication Date

October 2015

Volume

87

Issue

10

Start / End Page

1722 / 1726

Location

United States

Related Subject Headings

  • Young Adult
  • Virology
  • Severity of Illness Index
  • Real-Time Polymerase Chain Reaction
  • Oligonucleotides
  • Middle Aged
  • MicroRNAs
  • Male
  • Liver Cirrhosis
  • Liver