Skip to main content
Journal cover image

In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness

Publication ,  Conference
Wilson, JW; Degan, S; Gainey, CS; Deb, S; Dall, CP; Tameze-Rivas, Y; Zhang, J; Warren, WS
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
January 1, 2015

Pump-probe microscopy is a multiphoton technique that generates molecular contrast from absorptive pigments, such as melanin. It holds the potential to be used as a non-invasive screening tool to discern whether a given early-stage melanoma has acquired the capacity for metastasis. Here, we examined lesions in a Braf(V600E)-driven model of melanoma to assess whether loss of the tumor suppressor gene Pten in a is accompanied by a shift in pigment expression, as measured in vivo by pump-probe microscopy. The data were analyzed to determine differences in the excited-state lifetime of melanins expressed in Pten-competent and Pten-loss pigmented lesions. Loss of the tumor suppressor Pten was found to be accompanied by a statistically significant decrease in pixel-average excited state lifetime (p = 1.3e-4).

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414196

Publication Date

January 1, 2015

Volume

9329
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wilson, J. W., Degan, S., Gainey, C. S., Deb, S., Dall, C. P., Tameze-Rivas, Y., … Warren, W. S. (2015). In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9329). https://doi.org/10.1117/12.2079886
Wilson, J. W., S. Degan, C. S. Gainey, S. Deb, C. P. Dall, Y. Tameze-Rivas, J. Zhang, and W. S. Warren. “In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 9329, 2015. https://doi.org/10.1117/12.2079886.
Wilson JW, Degan S, Gainey CS, Deb S, Dall CP, Tameze-Rivas Y, et al. In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Wilson, J. W., et al. “In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9329, 2015. Scopus, doi:10.1117/12.2079886.
Wilson JW, Degan S, Gainey CS, Deb S, Dall CP, Tameze-Rivas Y, Zhang J, Warren WS. In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Journal cover image

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414196

Publication Date

January 1, 2015

Volume

9329