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Characterization of a Large Panel of Rabbit Monoclonal Antibodies against HIV-1 gp120 and Isolation of Novel Neutralizing Antibodies against the V3 Loop.

Publication ,  Journal Article
Qin, Y; Banerjee, S; Agrawal, A; Shi, H; Banasik, M; Lin, F; Rohl, K; LaBranche, C; Montefiori, DC; Cho, MW
Published in: PLoS One
2015

We recently reported the induction of potent, cross-clade neutralizing antibodies (nAbs) against Human Immunodeficiency Virus type-1 (HIV-1) in rabbits using gp120 based on an M-group consensus sequence. To better characterize these antibodies, 93 hybridomas were generated, which represent the largest panel of monoclonal antibodies (mAbs) ever generated from a vaccinated rabbit. The single most frequently recognized epitope of the isolated mAbs was at the very C-terminal end of the protein (APTKAKRRVVEREKR), followed by the V3 loop. A total of seven anti-V3 loop mAbs were isolated, two of which (10A3 and 10A37) exhibited neutralizing activity. In contrast to 10A3 and most other anti-V3 loop nAbs, 10A37 was atypical with its epitope positioned more towards the C-terminal half of the loop. To our knowledge, 10A37 is the most potent and broadly neutralizing anti-V3 loop mAb induced by vaccination. Interestingly, all seven anti-V3 loop mAbs competed with PGT121, suggesting a possibility that early induction of potent anti-V3 loop antibodies could prevent induction of more broadly neutralizing PGT121-like antibodies that target the conserved base of the V3 loop stem.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

6

Start / End Page

e0128823

Location

United States

Related Subject Headings

  • Vaccination
  • Rabbits
  • Protein Structure, Tertiary
  • Protein Structure, Secondary
  • Protein Binding
  • Neutralization Tests
  • Molecular Sequence Data
  • Models, Molecular
  • Hybridomas
  • HIV-1
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Qin, Y., Banerjee, S., Agrawal, A., Shi, H., Banasik, M., Lin, F., … Cho, M. W. (2015). Characterization of a Large Panel of Rabbit Monoclonal Antibodies against HIV-1 gp120 and Isolation of Novel Neutralizing Antibodies against the V3 Loop. PLoS One, 10(6), e0128823. https://doi.org/10.1371/journal.pone.0128823
Qin, Yali, Saikat Banerjee, Aditi Agrawal, Heliang Shi, Marisa Banasik, Feng Lin, Kari Rohl, Celia LaBranche, David C. Montefiori, and Michael W. Cho. “Characterization of a Large Panel of Rabbit Monoclonal Antibodies against HIV-1 gp120 and Isolation of Novel Neutralizing Antibodies against the V3 Loop.PLoS One 10, no. 6 (2015): e0128823. https://doi.org/10.1371/journal.pone.0128823.
Qin, Yali, et al. “Characterization of a Large Panel of Rabbit Monoclonal Antibodies against HIV-1 gp120 and Isolation of Novel Neutralizing Antibodies against the V3 Loop.PLoS One, vol. 10, no. 6, 2015, p. e0128823. Pubmed, doi:10.1371/journal.pone.0128823.
Qin Y, Banerjee S, Agrawal A, Shi H, Banasik M, Lin F, Rohl K, LaBranche C, Montefiori DC, Cho MW. Characterization of a Large Panel of Rabbit Monoclonal Antibodies against HIV-1 gp120 and Isolation of Novel Neutralizing Antibodies against the V3 Loop. PLoS One. 2015;10(6):e0128823.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

6

Start / End Page

e0128823

Location

United States

Related Subject Headings

  • Vaccination
  • Rabbits
  • Protein Structure, Tertiary
  • Protein Structure, Secondary
  • Protein Binding
  • Neutralization Tests
  • Molecular Sequence Data
  • Models, Molecular
  • Hybridomas
  • HIV-1