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Effect of cyclic strain on tensile properties of a naturally derived, decellularized tendon scaffold seeded with allogeneic tenocytes and associated messenger RNA expression.

Publication ,  Journal Article
Whitlock, PW; Seyler, TM; Northam, CN; Smith, TL; Poehling, GG; Koman, LA; Van Dyke, ME
Published in: J Surg Orthop Adv
2013

Naturally derived tendon scaffolds have the potential to improve the treatment of flexor tendon injuries. Seeded and unseeded tendon scaffolds were maintained in the presence or absence of physiologic strain for 7 days. After 7 days, the tensile properties and associated messenger RNA expression were compared. Seeded scaffolds maintained in the absence of strain had significantly lower tensile properties than unseeded tendons and fresh-frozen tendons. The loss of tensile properties was associated with elevated matrix metalloproteinase-2 and collagen III expression. Tensile properties of seeded scaffolds maintained in the presence of strain for 7 days after seeding did not differ from those of fresh-frozen tendons. This study demonstrates that the tensile properties of seeded, naturally derived tendon scaffolds will degrade rapidly in the absence of cyclic strain. Seeded scaffolds used for tendon reconstruction should be maintained under cyclic strain to maintain essential tensile properties.

Duke Scholars

Published In

J Surg Orthop Adv

DOI

ISSN

1548-825X

Publication Date

2013

Volume

22

Issue

3

Start / End Page

224 / 232

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Tensile Strength
  • Tendons
  • Tendon Injuries
  • RNA, Messenger
  • Humans
  • Equipment Design
  • Bioreactors
  • Allografts
 

Citation

APA
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ICMJE
MLA
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Whitlock, P. W., Seyler, T. M., Northam, C. N., Smith, T. L., Poehling, G. G., Koman, L. A., & Van Dyke, M. E. (2013). Effect of cyclic strain on tensile properties of a naturally derived, decellularized tendon scaffold seeded with allogeneic tenocytes and associated messenger RNA expression. J Surg Orthop Adv, 22(3), 224–232. https://doi.org/10.3113/jsoa.2013.0224
Whitlock, Patrick W., Thorsten M. Seyler, Casey N. Northam, Thomas L. Smith, Gary G. Poehling, L Andrew Koman, and Mark E. Van Dyke. “Effect of cyclic strain on tensile properties of a naturally derived, decellularized tendon scaffold seeded with allogeneic tenocytes and associated messenger RNA expression.J Surg Orthop Adv 22, no. 3 (2013): 224–32. https://doi.org/10.3113/jsoa.2013.0224.
Whitlock PW, Seyler TM, Northam CN, Smith TL, Poehling GG, Koman LA, et al. Effect of cyclic strain on tensile properties of a naturally derived, decellularized tendon scaffold seeded with allogeneic tenocytes and associated messenger RNA expression. J Surg Orthop Adv. 2013;22(3):224–32.
Whitlock, Patrick W., et al. “Effect of cyclic strain on tensile properties of a naturally derived, decellularized tendon scaffold seeded with allogeneic tenocytes and associated messenger RNA expression.J Surg Orthop Adv, vol. 22, no. 3, 2013, pp. 224–32. Pubmed, doi:10.3113/jsoa.2013.0224.
Whitlock PW, Seyler TM, Northam CN, Smith TL, Poehling GG, Koman LA, Van Dyke ME. Effect of cyclic strain on tensile properties of a naturally derived, decellularized tendon scaffold seeded with allogeneic tenocytes and associated messenger RNA expression. J Surg Orthop Adv. 2013;22(3):224–232.

Published In

J Surg Orthop Adv

DOI

ISSN

1548-825X

Publication Date

2013

Volume

22

Issue

3

Start / End Page

224 / 232

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Tissue Engineering
  • Tensile Strength
  • Tendons
  • Tendon Injuries
  • RNA, Messenger
  • Humans
  • Equipment Design
  • Bioreactors
  • Allografts