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A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires.

Publication ,  Journal Article
Chen, L; Carico, Z; Shih, H-Y; Krangel, MS
Published in: Nat Immunol
October 2015

The locus encoding the T cell antigen receptor (TCR) α-chain and δ-chain (Tcra-Tcrd) undergoes recombination of its variable-diversity-joining (V(D)J) segments in CD4(-)CD8(-) double-negative thymocytes and CD4(+)CD8(+) double-positive thymocytes to generate diverse TCRδ repertoires and TCRα repertoires, respectively. Here we identified a chromatin-interaction network in the Tcra-Tcrd locus in double-negative thymocytes that was formed by interactions between binding elements for the transcription factor CTCF. Disruption of a discrete chromatin loop encompassing the D, J and constant (C) segments of Tcrd allowed a single V segment to frequently contact and rearrange to D and J segments and dominate the adult TCRδ repertoire. Disruption of this loop also narrowed the TCRα repertoire, which, we believe, followed as a consequence of the restricted TCRδ repertoire. Hence, a single CTCF-mediated chromatin loop directly regulated TCRδ diversity and indirectly regulated TCRα diversity.

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Published In

Nat Immunol

DOI

EISSN

1529-2916

Publication Date

October 2015

Volume

16

Issue

10

Start / End Page

1085 / 1093

Location

United States

Related Subject Headings

  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Antigen, T-Cell, alpha-beta
  • Nucleic Acid Conformation
  • Mice
  • Immunology
  • Flow Cytometry
  • Chromatin
  • Animals
  • 3204 Immunology
  • 3101 Biochemistry and cell biology
 

Citation

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Chen, L., Carico, Z., Shih, H.-Y., & Krangel, M. S. (2015). A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires. Nat Immunol, 16(10), 1085–1093. https://doi.org/10.1038/ni.3232
Chen, Liang, Zachary Carico, Han-Yu Shih, and Michael S. Krangel. “A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires.Nat Immunol 16, no. 10 (October 2015): 1085–93. https://doi.org/10.1038/ni.3232.
Chen L, Carico Z, Shih H-Y, Krangel MS. A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires. Nat Immunol. 2015 Oct;16(10):1085–93.
Chen, Liang, et al. “A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires.Nat Immunol, vol. 16, no. 10, Oct. 2015, pp. 1085–93. Pubmed, doi:10.1038/ni.3232.
Chen L, Carico Z, Shih H-Y, Krangel MS. A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRδ and TCRα repertoires. Nat Immunol. 2015 Oct;16(10):1085–1093.

Published In

Nat Immunol

DOI

EISSN

1529-2916

Publication Date

October 2015

Volume

16

Issue

10

Start / End Page

1085 / 1093

Location

United States

Related Subject Headings

  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Antigen, T-Cell, alpha-beta
  • Nucleic Acid Conformation
  • Mice
  • Immunology
  • Flow Cytometry
  • Chromatin
  • Animals
  • 3204 Immunology
  • 3101 Biochemistry and cell biology