Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy.
Publication
, Journal Article
Hansen, AL; Nikolova, EN; Casiano-Negroni, A; Al-Hashimi, HM
Published in: J Am Chem Soc
March 25, 2009
We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.
Duke Scholars
Published In
J Am Chem Soc
DOI
EISSN
1520-5126
Publication Date
March 25, 2009
Volume
131
Issue
11
Start / End Page
3818 / 3819
Location
United States
Related Subject Headings
- RNA, Ribosomal
- Nucleic Acids
- Magnetic Resonance Spectroscopy
- Kinetics
- General Chemistry
- DNA Damage
- DNA
- Carbon Isotopes
- 40 Engineering
- 34 Chemical sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Hansen, A. L., Nikolova, E. N., Casiano-Negroni, A., & Al-Hashimi, H. M. (2009). Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy. J Am Chem Soc, 131(11), 3818–3819. https://doi.org/10.1021/ja8091399
Hansen, Alexandar L., Evgenia N. Nikolova, Anette Casiano-Negroni, and Hashim M. Al-Hashimi. “Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy.” J Am Chem Soc 131, no. 11 (March 25, 2009): 3818–19. https://doi.org/10.1021/ja8091399.
Hansen AL, Nikolova EN, Casiano-Negroni A, Al-Hashimi HM. Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy. J Am Chem Soc. 2009 Mar 25;131(11):3818–9.
Hansen, Alexandar L., et al. “Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy.” J Am Chem Soc, vol. 131, no. 11, Mar. 2009, pp. 3818–19. Pubmed, doi:10.1021/ja8091399.
Hansen AL, Nikolova EN, Casiano-Negroni A, Al-Hashimi HM. Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy. J Am Chem Soc. 2009 Mar 25;131(11):3818–3819.
Published In
J Am Chem Soc
DOI
EISSN
1520-5126
Publication Date
March 25, 2009
Volume
131
Issue
11
Start / End Page
3818 / 3819
Location
United States
Related Subject Headings
- RNA, Ribosomal
- Nucleic Acids
- Magnetic Resonance Spectroscopy
- Kinetics
- General Chemistry
- DNA Damage
- DNA
- Carbon Isotopes
- 40 Engineering
- 34 Chemical sciences