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Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo.

Publication ,  Journal Article
Jiang, Y; Gong, NN; Hu, XS; Ni, MJ; Pasi, R; Matsunami, H
Published in: Nat Neurosci
October 2015

The mammalian olfactory system uses a large family of odorant receptors (ORs) to detect and discriminate amongst a myriad of volatile odor molecules. Understanding odor coding requires comprehensive mapping between ORs and corresponding odors. We developed a means of high-throughput in vivo identification of OR repertoires responding to odorants using phosphorylated ribosome immunoprecipitation of mRNA from olfactory epithelium of odor-stimulated mice followed by RNA-Seq. This approach screened the endogenously expressed ORs against an odor in one set of experiments using awake and freely behaving mice. In combination with validations in a heterologous system, we identified sets of ORs for two odorants, acetophenone and 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), encompassing 69 OR-odorant pairs. We also identified shared amino acid residues specific to the acetophenone or TMT receptors and developed models to predict receptor activation by acetophenone. Our results provide a method for understanding the combinatorial coding of odors in vivo.

Duke Scholars

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Published In

Nat Neurosci

DOI

EISSN

1546-1726

Publication Date

October 2015

Volume

18

Issue

10

Start / End Page

1446 / 1454

Location

United States

Related Subject Headings

  • Smell
  • Receptors, Odorant
  • Polymerase Chain Reaction
  • Olfactory Receptor Neurons
  • Neurons
  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice
  • Male
  • In Situ Hybridization
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jiang, Y., Gong, N. N., Hu, X. S., Ni, M. J., Pasi, R., & Matsunami, H. (2015). Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo. Nat Neurosci, 18(10), 1446–1454. https://doi.org/10.1038/nn.4104
Jiang, Yue, Naihua Natalie Gong, Xiaoyang Serene Hu, Mengjue Jessica Ni, Radhika Pasi, and Hiroaki Matsunami. “Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo.Nat Neurosci 18, no. 10 (October 2015): 1446–54. https://doi.org/10.1038/nn.4104.
Jiang Y, Gong NN, Hu XS, Ni MJ, Pasi R, Matsunami H. Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo. Nat Neurosci. 2015 Oct;18(10):1446–54.
Jiang, Yue, et al. “Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo.Nat Neurosci, vol. 18, no. 10, Oct. 2015, pp. 1446–54. Pubmed, doi:10.1038/nn.4104.
Jiang Y, Gong NN, Hu XS, Ni MJ, Pasi R, Matsunami H. Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo. Nat Neurosci. 2015 Oct;18(10):1446–1454.

Published In

Nat Neurosci

DOI

EISSN

1546-1726

Publication Date

October 2015

Volume

18

Issue

10

Start / End Page

1446 / 1454

Location

United States

Related Subject Headings

  • Smell
  • Receptors, Odorant
  • Polymerase Chain Reaction
  • Olfactory Receptor Neurons
  • Neurons
  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice
  • Male
  • In Situ Hybridization