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Different G(i)-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils.

Publication ,  Journal Article
Berger, M; Budhu, S; Lu, E; Li, Y; Loike, D; Silverstein, SC; Loike, JD
Published in: J Leukoc Biol
May 2002

fMLP- or TNF-alpha-stimulated neutrophils produced H(2)O(2) when they adhered to fibrinogen-coated surfaces but not when they adhered to collagen I-, collagen IV-, or Matrigel-coated surfaces. In contrast, LTB4- or IL-8-stimulated neutrophils did not produce H(2)O(2) when they adhered to any of these surfaces. fMLP and TNF-alpha were much more potent than LTB4 and IL-8 in stimulating neutrophils to up-regulate and to activate their alpha(M)beta(2) integrins, as measured by the binding of specific monoclonal antibodies. Pretreatment of neutrophils with pertussis toxin completely blocked their production of H(2)O(2) on fibrinogen-coated surfaces in response to fMLP and their migration through Matrigel in response to fMLP, LTB4, and IL-8. These data show that although the fMLP, LTB4, and IL-8 receptors are coupled to pertussis toxin-sensitive Galpha proteins, they signal neutrophils to initiate qualitatively different effector functions. We propose that the qualitative differences in effector functions signaled by different chemoattractants reflect qualitative differences in using G-protein beta and/or gamma subunits or other factors by their cognate receptors.

Duke Scholars

Published In

J Leukoc Biol

ISSN

0741-5400

Publication Date

May 2002

Volume

71

Issue

5

Start / End Page

798 / 806

Location

England

Related Subject Headings

  • Virulence Factors, Bordetella
  • Signal Transduction
  • Receptors, Immunologic
  • Pertussis Toxin
  • Neutrophils
  • Kinetics
  • Immunology
  • Hydrogen Peroxide
  • Humans
  • Heterotrimeric GTP-Binding Proteins
 

Citation

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Berger, M., Budhu, S., Lu, E., Li, Y., Loike, D., Silverstein, S. C., & Loike, J. D. (2002). Different G(i)-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils. J Leukoc Biol, 71(5), 798–806.
Berger, Miles, Sadna Budhu, Emily Lu, Yongmei Li, Devora Loike, Samuel C. Silverstein, and John D. Loike. “Different G(i)-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils.J Leukoc Biol 71, no. 5 (May 2002): 798–806.
Berger M, Budhu S, Lu E, Li Y, Loike D, Silverstein SC, et al. Different G(i)-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils. J Leukoc Biol. 2002 May;71(5):798–806.
Berger, Miles, et al. “Different G(i)-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils.J Leukoc Biol, vol. 71, no. 5, May 2002, pp. 798–806.
Berger M, Budhu S, Lu E, Li Y, Loike D, Silverstein SC, Loike JD. Different G(i)-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils. J Leukoc Biol. 2002 May;71(5):798–806.

Published In

J Leukoc Biol

ISSN

0741-5400

Publication Date

May 2002

Volume

71

Issue

5

Start / End Page

798 / 806

Location

England

Related Subject Headings

  • Virulence Factors, Bordetella
  • Signal Transduction
  • Receptors, Immunologic
  • Pertussis Toxin
  • Neutrophils
  • Kinetics
  • Immunology
  • Hydrogen Peroxide
  • Humans
  • Heterotrimeric GTP-Binding Proteins