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Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.

Publication ,  Journal Article
Kulp, AJ; Sun, B; Ai, T; Manning, AJ; Orench-Rivera, N; Schmid, AK; Kuehn, MJ
Published in: PLoS One
2015

The production of outer membrane vesicles by Gram-negative bacteria has been well documented; however, the mechanism behind the biogenesis of these vesicles remains unclear. Here a high-throughput experimental method and systems-scale analysis was conducted to determine vesiculation values for the whole genome knockout library of Escherichia coli mutant strains (Keio collection). The resultant dataset quantitatively recapitulates previously observed phenotypes and implicates nearly 150 new genes in the process of vesiculation. Gene functional and biochemical pathway analyses suggest that mutations that truncate outer membrane structures such as lipopolysaccharide and enterobacterial common antigen lead to hypervesiculation, whereas mutants in oxidative stress response pathways result in lower levels. This study expands and refines the current knowledge regarding the cellular pathways required for outer membrane vesiculation in E. coli.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

9

Start / End Page

e0139200

Location

United States

Related Subject Headings

  • Phenotype
  • Mutation
  • Genome, Bacterial
  • General Science & Technology
  • Escherichia coli
  • Cytoplasmic Vesicles
  • Cell Membrane
  • Bacterial Outer Membrane Proteins
 

Citation

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Kulp, A. J., Sun, B., Ai, T., Manning, A. J., Orench-Rivera, N., Schmid, A. K., & Kuehn, M. J. (2015). Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli. PLoS One, 10(9), e0139200. https://doi.org/10.1371/journal.pone.0139200
Kulp, Adam J., Bo Sun, Teresa Ai, Andrew J. Manning, Nichole Orench-Rivera, Amy K. Schmid, and Meta J. Kuehn. “Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.PLoS One 10, no. 9 (2015): e0139200. https://doi.org/10.1371/journal.pone.0139200.
Kulp AJ, Sun B, Ai T, Manning AJ, Orench-Rivera N, Schmid AK, et al. Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli. PLoS One. 2015;10(9):e0139200.
Kulp, Adam J., et al. “Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.PLoS One, vol. 10, no. 9, 2015, p. e0139200. Pubmed, doi:10.1371/journal.pone.0139200.
Kulp AJ, Sun B, Ai T, Manning AJ, Orench-Rivera N, Schmid AK, Kuehn MJ. Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli. PLoS One. 2015;10(9):e0139200.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

9

Start / End Page

e0139200

Location

United States

Related Subject Headings

  • Phenotype
  • Mutation
  • Genome, Bacterial
  • General Science & Technology
  • Escherichia coli
  • Cytoplasmic Vesicles
  • Cell Membrane
  • Bacterial Outer Membrane Proteins