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Teratogenic, bioenergetic, and behavioral effects of exposure to total particulate matter on early development of zebrafish (Danio rerio) are not mimicked by nicotine.

Publication ,  Journal Article
Massarsky, A; Jayasundara, N; Bailey, JM; Oliveri, AN; Levin, ED; Prasad, GL; Di Giulio, RT
Published in: Neurotoxicol Teratol
2015

Cigarette smoke has been associated with a number of pathologies; however, the mechanisms leading to developmental effects are yet to be fully understood. The zebrafish embryo is regarded as a 'bridge model'; however, not many studies examined its applicability to cigarette smoke toxicity. This study examined the effects of total particulate matter (TPM) from 3R4F reference cigarettes on the early development of zebrafish (Danio rerio). Zebrafish embryos were exposed to two concentrations of TPM (0.4 and 1.4 μg/mL equi-nicotine units) or nicotine at equivalent doses. The exposures began at 2h post-fertilization (hpf) and lasted until 96 hpf. Several physiological parameters were assessed during or after the exposure. We show that TPM increased mortality, delayed hatching, and increased the incidence of deformities in zebrafish. TPM exposure also increased the incidence of hemorrhage and disrupted the angiogenesis of the major vessels in the brain. Moreover, TPM exposure reduced the larval body length, decreased the heart rate, and reduced the metabolic rate. Biomarkers of xenobiotic metabolism and oxidative stress were also affected. TPM-exposed zebrafish also differed behaviorally: at 24 hpf the embryos had a higher frequency of spontaneous contractions and at 144 hpf the larvae displayed swimming hyperactivity. This study demonstrates that TPM disrupts several aspects of early development in zebrafish. The effects reported for TPM were not attributable to nicotine, since embryos treated with nicotine alone did not differ significantly from the control group. Collectively, our work illustrates the utility of zebrafish as an alternative model to evaluate the toxic effects of cigarette smoke constituents.

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Published In

Neurotoxicol Teratol

DOI

EISSN

1872-9738

Publication Date

2015

Volume

51

Start / End Page

77 / 88

Location

United States

Related Subject Headings

  • Zebrafish
  • Xenobiotics
  • Toxicology
  • Teratogens
  • Particulate Matter
  • Oxidative Stress
  • Nicotinic Agonists
  • Nicotine
  • Neovascularization, Physiologic
  • Hemoglobins
 

Citation

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ICMJE
MLA
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Massarsky, A., Jayasundara, N., Bailey, J. M., Oliveri, A. N., Levin, E. D., Prasad, G. L., & Di Giulio, R. T. (2015). Teratogenic, bioenergetic, and behavioral effects of exposure to total particulate matter on early development of zebrafish (Danio rerio) are not mimicked by nicotine. Neurotoxicol Teratol, 51, 77–88. https://doi.org/10.1016/j.ntt.2015.09.006
Massarsky, Andrey, Nishad Jayasundara, Jordan M. Bailey, Anthony N. Oliveri, Edward D. Levin, G. L. Prasad, and Richard T. Di Giulio. “Teratogenic, bioenergetic, and behavioral effects of exposure to total particulate matter on early development of zebrafish (Danio rerio) are not mimicked by nicotine.Neurotoxicol Teratol 51 (2015): 77–88. https://doi.org/10.1016/j.ntt.2015.09.006.
Massarsky A, Jayasundara N, Bailey JM, Oliveri AN, Levin ED, Prasad GL, et al. Teratogenic, bioenergetic, and behavioral effects of exposure to total particulate matter on early development of zebrafish (Danio rerio) are not mimicked by nicotine. Neurotoxicol Teratol. 2015;51:77–88.
Massarsky, Andrey, et al. “Teratogenic, bioenergetic, and behavioral effects of exposure to total particulate matter on early development of zebrafish (Danio rerio) are not mimicked by nicotine.Neurotoxicol Teratol, vol. 51, 2015, pp. 77–88. Pubmed, doi:10.1016/j.ntt.2015.09.006.
Massarsky A, Jayasundara N, Bailey JM, Oliveri AN, Levin ED, Prasad GL, Di Giulio RT. Teratogenic, bioenergetic, and behavioral effects of exposure to total particulate matter on early development of zebrafish (Danio rerio) are not mimicked by nicotine. Neurotoxicol Teratol. 2015;51:77–88.
Journal cover image

Published In

Neurotoxicol Teratol

DOI

EISSN

1872-9738

Publication Date

2015

Volume

51

Start / End Page

77 / 88

Location

United States

Related Subject Headings

  • Zebrafish
  • Xenobiotics
  • Toxicology
  • Teratogens
  • Particulate Matter
  • Oxidative Stress
  • Nicotinic Agonists
  • Nicotine
  • Neovascularization, Physiologic
  • Hemoglobins