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Identification of HIV-1 genitourinary tract compartmentalization by analyzing the env gene sequences in urine.

Publication ,  Journal Article
Blasi, M; Carpenter, JH; Balakumaran, B; Cara, A; Gao, F; Klotman, ME
Published in: AIDS
August 24, 2015

OBJECTIVE: HIV-1 persists indefinitely in memory CD4 T cells and other long-lived cellular reservoirs despite antiretroviral therapy. Our group had previously demonstrated that HIV-1 can establish a productive infection in renal epithelial cells and that the kidney represents a separate compartment for HIV-1 replication. Here, to better understand the viruses in this unique site, we genetically characterized and compared the viruses in blood and urine specimens from 24 HIV-1 infected patients with detectable viremia. DESIGN AND METHODS: Blood and urine samples were obtained from 35 HIV-1 positive patients. Single-genome amplification was performed on HIV-1 env RNA and DNA isolated from urine supernatants and urine-derived cell pellets, respectively, as well as from plasma and peripheral blood mononuclear cell from the same individuals. Neighbor-joining trees were constructed under the Kimura 2-parameter model. RESULTS: We amplified and sequenced the full-length HIV-1 envelope (env) gene from 12 of the 24 individuals, indicating that 50% of the viremic HIV-1-positive patients had viral RNA in their urine. Phylogenetic analysis of the env sequences from four individuals with more than 15 urine-derived env sequences showed that the majority of the sequences from urine formed distinct cluster(s) independent of those peripheral blood mononuclear cell and plasma-derived sequences, consistent with viral compartmentalization in the urine. CONCLUSION: Our results suggest the presence of a distinct HIV compartment in the genitourinary tract.

Duke Scholars

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Published In

AIDS

DOI

EISSN

1473-5571

Publication Date

August 24, 2015

Volume

29

Issue

13

Start / End Page

1651 / 1657

Location

England

Related Subject Headings

  • Virology
  • Urine
  • Urinary Tract Infections
  • Sequence Analysis, DNA
  • RNA, Viral
  • Polymerase Chain Reaction
  • Male
  • Leukocytes, Mononuclear
  • Humans
  • HIV-1
 

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Blasi, M., Carpenter, J. H., Balakumaran, B., Cara, A., Gao, F., & Klotman, M. E. (2015). Identification of HIV-1 genitourinary tract compartmentalization by analyzing the env gene sequences in urine. AIDS, 29(13), 1651–1657. https://doi.org/10.1097/QAD.0000000000000757
Blasi, Maria, J Harris Carpenter, Bala Balakumaran, Andrea Cara, Feng Gao, and Mary E. Klotman. “Identification of HIV-1 genitourinary tract compartmentalization by analyzing the env gene sequences in urine.AIDS 29, no. 13 (August 24, 2015): 1651–57. https://doi.org/10.1097/QAD.0000000000000757.
Blasi M, Carpenter JH, Balakumaran B, Cara A, Gao F, Klotman ME. Identification of HIV-1 genitourinary tract compartmentalization by analyzing the env gene sequences in urine. AIDS. 2015 Aug 24;29(13):1651–7.
Blasi, Maria, et al. “Identification of HIV-1 genitourinary tract compartmentalization by analyzing the env gene sequences in urine.AIDS, vol. 29, no. 13, Aug. 2015, pp. 1651–57. Pubmed, doi:10.1097/QAD.0000000000000757.
Blasi M, Carpenter JH, Balakumaran B, Cara A, Gao F, Klotman ME. Identification of HIV-1 genitourinary tract compartmentalization by analyzing the env gene sequences in urine. AIDS. 2015 Aug 24;29(13):1651–1657.

Published In

AIDS

DOI

EISSN

1473-5571

Publication Date

August 24, 2015

Volume

29

Issue

13

Start / End Page

1651 / 1657

Location

England

Related Subject Headings

  • Virology
  • Urine
  • Urinary Tract Infections
  • Sequence Analysis, DNA
  • RNA, Viral
  • Polymerase Chain Reaction
  • Male
  • Leukocytes, Mononuclear
  • Humans
  • HIV-1