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Identification of a common epitope between enterovirus 71 and human MED25 proteins which may explain virus-associated neurological disease.

Publication ,  Journal Article
Fan, P; Li, X; Sun, S; Su, W; An, D; Gao, F; Kong, W; Jiang, C
Published in: Viruses
March 27, 2015

Enterovirus 71 (EV71) is a major causative pathogen of hand, foot and mouth disease with especially severe neurologic complications, which mainly account for fatalities from this disease. To date, the pathogenesis of EV71 in the central neurons system has remained unclear. Cytokine-mediated immunopathogenesis and nervous tissue damage by virus proliferation are two widely speculated causes of the neurological disease. To further study the pathogenesis, we identified a common epitope (co-epitope) between EV71 VP1 and human mediator complex subunit 25 (MED25) highly expressed in brain stem. A monoclonal antibody (2H2) against the co-epitope was prepared, and its interaction with MED25 was examined by ELISA, immunofluorescence assay and Western blot in vitro and by live small animal imaging in vivo. Additionally, 2H2 could bind to both VP1 and MED25 with the affinity constant (Kd) of 10-7 M as determined by the ForteBio Octet System. Intravenously injected 2H2 was distributed in brain stem of mice after seven days of EV71 infection. Interestingly, 2H2-like antibodies were detected in the serum of EV71-infected patients. These findings suggest that EV71 infection induces the production of antibodies that can bind to autoantigens expressed in nervous tissue and maybe further trigger autoimmune reactions resulting in neurological disease.

Duke Scholars

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Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

March 27, 2015

Volume

7

Issue

4

Start / End Page

1558 / 1577

Location

Switzerland

Related Subject Headings

  • Whole Body Imaging
  • Viral Structural Proteins
  • Nervous System Diseases
  • Mice
  • Mediator Complex
  • Humans
  • Fluorescent Antibody Technique
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • Enterovirus Infections
 

Citation

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Fan, P., Li, X., Sun, S., Su, W., An, D., Gao, F., … Jiang, C. (2015). Identification of a common epitope between enterovirus 71 and human MED25 proteins which may explain virus-associated neurological disease. Viruses, 7(4), 1558–1577. https://doi.org/10.3390/v7041558
Fan, Peihu, Xiaojun Li, Shiyang Sun, Weiheng Su, Dong An, Feng Gao, Wei Kong, and Chunlai Jiang. “Identification of a common epitope between enterovirus 71 and human MED25 proteins which may explain virus-associated neurological disease.Viruses 7, no. 4 (March 27, 2015): 1558–77. https://doi.org/10.3390/v7041558.
Fan, Peihu, et al. “Identification of a common epitope between enterovirus 71 and human MED25 proteins which may explain virus-associated neurological disease.Viruses, vol. 7, no. 4, Mar. 2015, pp. 1558–77. Pubmed, doi:10.3390/v7041558.
Fan P, Li X, Sun S, Su W, An D, Gao F, Kong W, Jiang C. Identification of a common epitope between enterovirus 71 and human MED25 proteins which may explain virus-associated neurological disease. Viruses. 2015 Mar 27;7(4):1558–1577.

Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

March 27, 2015

Volume

7

Issue

4

Start / End Page

1558 / 1577

Location

Switzerland

Related Subject Headings

  • Whole Body Imaging
  • Viral Structural Proteins
  • Nervous System Diseases
  • Mice
  • Mediator Complex
  • Humans
  • Fluorescent Antibody Technique
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • Enterovirus Infections