Practical methods for in vivo cortical physiology with 2-photon microscopy and bulk loading of fluorescent calcium indicator dyes
Publication
, Journal Article
Hooser, SDV; Johnson, EN; Li, Y; Mazurek, M; Culp, JH; Roy, A; Kasliwal, R; Flavahan, K
January 1, 2015
In vivo 2-photon imaging of neurons that have been bulk-loaded with fl uorescent calcium indicator dyes is permitting many fundamental principles of neural circuit organization and development to be uncovered. In this article, we describe the materials and procedures that we have used in our investigations of ferrets, tree shrews, and mice. Special attention is given to the design and construction of custom stereotaxic devices and the prevention of stray light from entering the 2-photon microscope during vision experiments.
Duke Scholars
Citation
APA
Chicago
ICMJE
MLA
NLM
Hooser, S. D. V., Johnson, E. N., Li, Y., Mazurek, M., Culp, J. H., Roy, A., … Flavahan, K. (2015). Practical methods for in vivo cortical physiology with 2-photon microscopy and bulk loading of fluorescent calcium indicator dyes, 117–141. https://doi.org/10.1007/978-1-4939-1963-5_6
Hooser, S. D. V., E. N. Johnson, Y. Li, M. Mazurek, J. H. Culp, A. Roy, R. Kasliwal, and K. Flavahan. “Practical methods for in vivo cortical physiology with 2-photon microscopy and bulk loading of fluorescent calcium indicator dyes,” January 1, 2015, 117–41. https://doi.org/10.1007/978-1-4939-1963-5_6.
Hooser SDV, Johnson EN, Li Y, Mazurek M, Culp JH, Roy A, et al. Practical methods for in vivo cortical physiology with 2-photon microscopy and bulk loading of fluorescent calcium indicator dyes. 2015 Jan 1;117–41.
Hooser, S. D. V., et al. Practical methods for in vivo cortical physiology with 2-photon microscopy and bulk loading of fluorescent calcium indicator dyes. Jan. 2015, pp. 117–41. Scopus, doi:10.1007/978-1-4939-1963-5_6.
Hooser SDV, Johnson EN, Li Y, Mazurek M, Culp JH, Roy A, Kasliwal R, Flavahan K. Practical methods for in vivo cortical physiology with 2-photon microscopy and bulk loading of fluorescent calcium indicator dyes. 2015 Jan 1;117–141.