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Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.

Publication ,  Journal Article
Zhang, G; Cai, F; de Rivera, IL; Zhou, Z; Zhang, J; Nkengasong, J; Gao, F; Yang, C
Published in: J Clin Microbiol
January 2016

A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing.

Duke Scholars

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

January 2016

Volume

54

Issue

1

Start / End Page

220 / 222

Location

United States

Related Subject Headings

  • Specimen Handling
  • Mutation, Missense
  • Microbiology
  • Microbial Sensitivity Tests
  • Humans
  • HIV-1
  • HIV Infections
  • Genotyping Techniques
  • Drug Resistance, Viral
  • Desiccation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, G., Cai, F., de Rivera, I. L., Zhou, Z., Zhang, J., Nkengasong, J., … Yang, C. (2016). Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay. J Clin Microbiol, 54(1), 220–222. https://doi.org/10.1128/JCM.02833-15
Zhang, Guoqing, Fangping Cai, Ivette Lorenzana de Rivera, Zhiyong Zhou, Jing Zhang, John Nkengasong, Feng Gao, and Chunfu Yang. “Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.J Clin Microbiol 54, no. 1 (January 2016): 220–22. https://doi.org/10.1128/JCM.02833-15.
Zhang G, Cai F, de Rivera IL, Zhou Z, Zhang J, Nkengasong J, et al. Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay. J Clin Microbiol. 2016 Jan;54(1):220–2.
Zhang, Guoqing, et al. “Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.J Clin Microbiol, vol. 54, no. 1, Jan. 2016, pp. 220–22. Pubmed, doi:10.1128/JCM.02833-15.
Zhang G, Cai F, de Rivera IL, Zhou Z, Zhang J, Nkengasong J, Gao F, Yang C. Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay. J Clin Microbiol. 2016 Jan;54(1):220–222.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

January 2016

Volume

54

Issue

1

Start / End Page

220 / 222

Location

United States

Related Subject Headings

  • Specimen Handling
  • Mutation, Missense
  • Microbiology
  • Microbial Sensitivity Tests
  • Humans
  • HIV-1
  • HIV Infections
  • Genotyping Techniques
  • Drug Resistance, Viral
  • Desiccation