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Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics.

Publication ,  Journal Article
Maddala, R; Walters, M; Brophy, PJ; Bennett, V; Rao, PV
Published in: Am J Physiol Cell Physiol
January 15, 2016

Periaxin (Prx), a PDZ domain protein expressed preferentially in myelinating Schwann cells and lens fibers, plays a key role in membrane scaffolding and cytoarchitecture. Little is known, however, about how Prx is anchored to the plasma membrane. Here we report that ankyrin-B (AnkB), a well-characterized adaptor protein involved in linking the spectrin-actin cytoskeleton to integral membrane proteins, is required for membrane association of Prx in lens fibers and colocalizes with Prx in hexagonal fiber cells. Under AnkB haploinsufficiency, Prx accumulates in the soluble fraction with a concomitant loss from the membrane-enriched fraction of mouse lenses. Moreover, AnkB haploinsufficiency induced age-dependent disruptions in fiber cell hexagonal geometry and radial alignment and decreased compressive stiffness in mouse lenses parallel to the changes observed in Prx null mouse lens. Both AnkB- and Prx-deficient mice exhibit disruptions in membrane organization of the spectrin-actin network and the dystrophin-glycoprotein complex in lens fiber cells. Taken together, these observations reveal that AnkB is required for Prx membrane anchoring and for maintenance of lens fiber cell hexagonal geometry, membrane skeleton organization, and biomechanics.

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Published In

Am J Physiol Cell Physiol

DOI

EISSN

1522-1563

Publication Date

January 15, 2016

Volume

310

Issue

2

Start / End Page

C115 / C126

Location

United States

Related Subject Headings

  • Tensile Strength
  • Stress, Mechanical
  • Protein Binding
  • Physiology
  • Mice, Knockout
  • Mice
  • Membrane Proteins
  • Lens, Crystalline
  • In Vitro Techniques
  • Hardness
 

Citation

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Maddala, R., Walters, M., Brophy, P. J., Bennett, V., & Rao, P. V. (2016). Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics. Am J Physiol Cell Physiol, 310(2), C115–C126. https://doi.org/10.1152/ajpcell.00111.2015
Maddala, Rupalatha, Mark Walters, Peter J. Brophy, Vann Bennett, and Ponugoti V. Rao. “Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics.Am J Physiol Cell Physiol 310, no. 2 (January 15, 2016): C115–26. https://doi.org/10.1152/ajpcell.00111.2015.
Maddala R, Walters M, Brophy PJ, Bennett V, Rao PV. Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics. Am J Physiol Cell Physiol. 2016 Jan 15;310(2):C115–26.
Maddala, Rupalatha, et al. “Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics.Am J Physiol Cell Physiol, vol. 310, no. 2, Jan. 2016, pp. C115–26. Pubmed, doi:10.1152/ajpcell.00111.2015.
Maddala R, Walters M, Brophy PJ, Bennett V, Rao PV. Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics. Am J Physiol Cell Physiol. 2016 Jan 15;310(2):C115–C126.

Published In

Am J Physiol Cell Physiol

DOI

EISSN

1522-1563

Publication Date

January 15, 2016

Volume

310

Issue

2

Start / End Page

C115 / C126

Location

United States

Related Subject Headings

  • Tensile Strength
  • Stress, Mechanical
  • Protein Binding
  • Physiology
  • Mice, Knockout
  • Mice
  • Membrane Proteins
  • Lens, Crystalline
  • In Vitro Techniques
  • Hardness