Skip to main content
Journal cover image

Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins.

Publication ,  Journal Article
Heaton, NS; Sachs, D; Chen, C-J; Hai, R; Palese, P
Published in: Proc Natl Acad Sci U S A
December 10, 2013

The molecular basis for the diversity across influenza strains is poorly understood. To gain insight into this question, we mutagenized the viral genome and sequenced recoverable viruses. Only two small regions in the genome were enriched for insertions, the hemagglutinin head and the immune-modulatory nonstructural protein 1. These proteins play a major role in host adaptation, and thus need to be able to evolve rapidly. We propose a model in which certain influenza A virus proteins (or protein domains) exist as highly plastic scaffolds, which will readily accept mutations yet retain their functionality. This model implies that the ability to rapidly acquire mutations is an inherent aspect of influenza HA and nonstructural protein 1 proteins; further, this may explain why rapid antigenic drift and a broad host range is observed with influenza A virus and not with some other RNA viruses.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 10, 2013

Volume

110

Issue

50

Start / End Page

20248 / 20253

Location

United States

Related Subject Headings

  • Viral Nonstructural Proteins
  • Sequence Analysis, DNA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Mutagenesis
  • Molecular Sequence Data
  • Models, Genetic
  • Madin Darby Canine Kidney Cells
  • Influenza A virus
  • Humans
  • Hemagglutinins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Heaton, N. S., Sachs, D., Chen, C.-J., Hai, R., & Palese, P. (2013). Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins. Proc Natl Acad Sci U S A, 110(50), 20248–20253. https://doi.org/10.1073/pnas.1320524110
Heaton, Nicholas S., David Sachs, Chi-Jene Chen, Rong Hai, and Peter Palese. “Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins.Proc Natl Acad Sci U S A 110, no. 50 (December 10, 2013): 20248–53. https://doi.org/10.1073/pnas.1320524110.
Heaton NS, Sachs D, Chen C-J, Hai R, Palese P. Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20248–53.
Heaton, Nicholas S., et al. “Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins.Proc Natl Acad Sci U S A, vol. 110, no. 50, Dec. 2013, pp. 20248–53. Pubmed, doi:10.1073/pnas.1320524110.
Heaton NS, Sachs D, Chen C-J, Hai R, Palese P. Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20248–20253.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 10, 2013

Volume

110

Issue

50

Start / End Page

20248 / 20253

Location

United States

Related Subject Headings

  • Viral Nonstructural Proteins
  • Sequence Analysis, DNA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Mutagenesis
  • Molecular Sequence Data
  • Models, Genetic
  • Madin Darby Canine Kidney Cells
  • Influenza A virus
  • Humans
  • Hemagglutinins