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Integration Site Choice of a Feline Immunodeficiency Virus Vector

Publication ,  Journal Article
Kang, Y; Moressi, CJ; Scheetz, TE; Xie, L; Tran, DT; Casavant, TL; Ak, P; Benham, CJ; Davidson, BL; McCray, PB
Published in: Journal of Virology
September 2006

We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a “bendable” structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

Duke Scholars

Published In

Journal of Virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

September 2006

Volume

80

Issue

17

Start / End Page

8820 / 8823

Publisher

American Society for Microbiology

Related Subject Headings

  • Virology
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

Citation

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Kang, Y., Moressi, C. J., Scheetz, T. E., Xie, L., Tran, D. T., Casavant, T. L., … McCray, P. B. (2006). Integration Site Choice of a Feline Immunodeficiency Virus Vector. Journal of Virology, 80(17), 8820–8823. https://doi.org/10.1128/jvi.00719-06
Kang, Yubin, Christopher J. Moressi, Todd E. Scheetz, Litao Xie, Diane Thi Tran, Thomas L. Casavant, Prashanth Ak, Craig J. Benham, Beverly L. Davidson, and Paul B. McCray. “Integration Site Choice of a Feline Immunodeficiency Virus Vector.” Journal of Virology 80, no. 17 (September 2006): 8820–23. https://doi.org/10.1128/jvi.00719-06.
Kang Y, Moressi CJ, Scheetz TE, Xie L, Tran DT, Casavant TL, et al. Integration Site Choice of a Feline Immunodeficiency Virus Vector. Journal of Virology. 2006 Sep;80(17):8820–3.
Kang, Yubin, et al. “Integration Site Choice of a Feline Immunodeficiency Virus Vector.” Journal of Virology, vol. 80, no. 17, American Society for Microbiology, Sept. 2006, pp. 8820–23. Crossref, doi:10.1128/jvi.00719-06.
Kang Y, Moressi CJ, Scheetz TE, Xie L, Tran DT, Casavant TL, Ak P, Benham CJ, Davidson BL, McCray PB. Integration Site Choice of a Feline Immunodeficiency Virus Vector. Journal of Virology. American Society for Microbiology; 2006 Sep;80(17):8820–8823.

Published In

Journal of Virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

September 2006

Volume

80

Issue

17

Start / End Page

8820 / 8823

Publisher

American Society for Microbiology

Related Subject Headings

  • Virology
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences