Skip to main content
Journal cover image

Induction of hyporesponsiveness to intact foreign protein via retroviral-mediated gene expression: The IgG scaffold is important for induction and maintenance of immune hyporesponsiveness

Publication ,  Journal Article
Kang, Y; Melo, M; Deng, E; Tisch, R; El-Amine, M; Scott, DW
Published in: Proceedings of the National Academy of Sciences
July 20, 1999

IgG molecules can be highly tolerogenic carriers for associated antigens. Previously, we reported that recipients of bone marrow or lipopolysaccharide-stimulated B-cell blasts, both of which were retrovirally gene-transferred with an immunodominant peptide in-frame with the variable region of a murine IgG heavy chain, were rendered profoundly unresponsive to that epitope. To further investigate whether tolerance to larger molecules can be achieved via this approach and whether the IgG scaffold is important for induction and maintenance of immunological tolerance, we engineered two retroviral constructs encoding the cI λ repressor (MBAE-1–102 and MBAE-1–102-IgG) for gene transfer. Our results show that recipients of bone marrow or peripheral B cells, transduced with the MBAE-1–102-IgG recombinant, are hyporesponsive to p1–102. In addition, the self-IgG scaffold enhanced the induction and maintenance of such an immune hyporesponsiveness. Thus, our studies demonstrate that-expressed IgG heavy chain fusion protein can be processed and presented on the appropriate MHC class II, resulting in hyporesponsiveness to that antigen and offering an additional therapeutic approach to autoimmune diseases.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proceedings of the National Academy of Sciences

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

July 20, 1999

Volume

96

Issue

15

Start / End Page

8609 / 8614

Publisher

Proceedings of the National Academy of Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kang, Y., Melo, M., Deng, E., Tisch, R., El-Amine, M., & Scott, D. W. (1999). Induction of hyporesponsiveness to intact foreign protein via retroviral-mediated gene expression: The IgG scaffold is important for induction and maintenance of immune hyporesponsiveness. Proceedings of the National Academy of Sciences, 96(15), 8609–8614. https://doi.org/10.1073/pnas.96.15.8609
Kang, Yubin, Marco Melo, Edward Deng, Roland Tisch, Moustapha El-Amine, and David W. Scott. “Induction of hyporesponsiveness to intact foreign protein via retroviral-mediated gene expression: The IgG scaffold is important for induction and maintenance of immune hyporesponsiveness.” Proceedings of the National Academy of Sciences 96, no. 15 (July 20, 1999): 8609–14. https://doi.org/10.1073/pnas.96.15.8609.
Kang Y, Melo M, Deng E, Tisch R, El-Amine M, Scott DW. Induction of hyporesponsiveness to intact foreign protein via retroviral-mediated gene expression: The IgG scaffold is important for induction and maintenance of immune hyporesponsiveness. Proceedings of the National Academy of Sciences. 1999 Jul 20;96(15):8609–14.
Kang, Yubin, et al. “Induction of hyporesponsiveness to intact foreign protein via retroviral-mediated gene expression: The IgG scaffold is important for induction and maintenance of immune hyporesponsiveness.” Proceedings of the National Academy of Sciences, vol. 96, no. 15, Proceedings of the National Academy of Sciences, July 1999, pp. 8609–14. Crossref, doi:10.1073/pnas.96.15.8609.
Kang Y, Melo M, Deng E, Tisch R, El-Amine M, Scott DW. Induction of hyporesponsiveness to intact foreign protein via retroviral-mediated gene expression: The IgG scaffold is important for induction and maintenance of immune hyporesponsiveness. Proceedings of the National Academy of Sciences. Proceedings of the National Academy of Sciences; 1999 Jul 20;96(15):8609–8614.
Journal cover image

Published In

Proceedings of the National Academy of Sciences

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

July 20, 1999

Volume

96

Issue

15

Start / End Page

8609 / 8614

Publisher

Proceedings of the National Academy of Sciences