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Remodeling Tissue Interfaces and the Thermodynamics of Zipping during Dorsal Closure in Drosophila.

Publication ,  Journal Article
Lu, H; Sokolow, A; Kiehart, DP; Edwards, GS
Published in: Biophysical journal
December 2015

Dorsal closure during Drosophila embryogenesis is an important model system for investigating the biomechanics of morphogenesis. During closure, two flanks of lateral epidermis (with actomyosin-rich purse strings near each leading edge) close an eye-shaped opening that is filled with amnioserosa. At each canthus (corner of the eye) a zipping process remodels the tissue interfaces between the leading edges of the lateral epidermis and the amnioserosa. We investigated zipping dynamics and found that apposing leading edge cells come together at their apical ends and then square off basally to form a lateral junction. Meanwhile, the purse strings act as contractile elastic rods bent toward the embryo interior near each canthus. We propose that a canthus-localized force contributes to both bending the ends of the purse strings and the formation of lateral junctions. We developed a thermodynamic model for zipping based on three-dimensional remodeling of the tissue interfaces and the reaction dynamics of adhesion molecules in junctions and elsewhere, which we applied to zipping during unperturbed wild-type closure and to laser or genetically perturbed closure. We identified two processes that can contribute to the zipping mechanism, consistent with experiments, distinguished by whether amnioserosa dynamics do or do not augment canthus adhesion dynamics.

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Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

December 2015

Volume

109

Issue

11

Start / End Page

2406 / 2417

Related Subject Headings

  • Thermodynamics
  • Models, Biological
  • Mechanical Phenomena
  • Lasers
  • Embryonic Development
  • Drosophila melanogaster
  • Biophysics
  • Biomechanical Phenomena
  • Animals
  • 51 Physical sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Lu, H., Sokolow, A., Kiehart, D. P., & Edwards, G. S. (2015). Remodeling Tissue Interfaces and the Thermodynamics of Zipping during Dorsal Closure in Drosophila. Biophysical Journal, 109(11), 2406–2417. https://doi.org/10.1016/j.bpj.2015.10.017
Lu, Heng, Adam Sokolow, Daniel P. Kiehart, and Glenn S. Edwards. “Remodeling Tissue Interfaces and the Thermodynamics of Zipping during Dorsal Closure in Drosophila.Biophysical Journal 109, no. 11 (December 2015): 2406–17. https://doi.org/10.1016/j.bpj.2015.10.017.
Lu H, Sokolow A, Kiehart DP, Edwards GS. Remodeling Tissue Interfaces and the Thermodynamics of Zipping during Dorsal Closure in Drosophila. Biophysical journal. 2015 Dec;109(11):2406–17.
Lu, Heng, et al. “Remodeling Tissue Interfaces and the Thermodynamics of Zipping during Dorsal Closure in Drosophila.Biophysical Journal, vol. 109, no. 11, Dec. 2015, pp. 2406–17. Epmc, doi:10.1016/j.bpj.2015.10.017.
Lu H, Sokolow A, Kiehart DP, Edwards GS. Remodeling Tissue Interfaces and the Thermodynamics of Zipping during Dorsal Closure in Drosophila. Biophysical journal. 2015 Dec;109(11):2406–2417.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

December 2015

Volume

109

Issue

11

Start / End Page

2406 / 2417

Related Subject Headings

  • Thermodynamics
  • Models, Biological
  • Mechanical Phenomena
  • Lasers
  • Embryonic Development
  • Drosophila melanogaster
  • Biophysics
  • Biomechanical Phenomena
  • Animals
  • 51 Physical sciences