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Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming.

Publication ,  Journal Article
Fan, Y; Marcy, G; Lee, ESM; Rozen, S; Mattar, CNZ; Waddington, SN; Goh, ELK; Choolani, M; Chan, JKY
Published in: PLoS One
2014

Neural stem/progenitor cells (NSC) have the potential for treatment of a wide range of neurological diseases such as Parkinson Disease and multiple sclerosis. Currently, NSC have been isolated only from hippocampus and subventricular zone (SVZ) of the adult brain. It is not known whether NSC can be found in all parts of the developing mid-trimester central nervous system (CNS) when the brain undergoes massive transformation and growth. Multipotent NSC from the mid-trimester cerebra, thalamus, SVZ, hippocampus, thalamus, cerebellum, brain stem and spinal cord can be derived and propagated as clonal neurospheres with increasing frequencies with increasing gestations. These NSC can undergo multi-lineage differentiation both in vitro and in vivo, and engraft in a developmental murine model. Regionally-derived NSC are phenotypically distinct, with hippocampal NSC having a significantly higher neurogenic potential (53.6%) over other sources (range of 0%-27.5%, p<0.004). Whole genome expression analysis showed differential gene expression between these regionally-derived NSC, which involved the Notch, epidermal growth factor as well as interleukin pathways. We have shown the presence of phenotypically-distinct regionally-derived NSC from the mid-trimester CNS, which may reflect the ontological differences occurring within the CNS. Aside from informing on the role of such cells during fetal growth, they may be useful for different cellular therapy applications.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

9

Start / End Page

e105985

Location

United States

Related Subject Headings

  • Stem Cell Transplantation
  • Spheroids, Cellular
  • Pregnancy Trimester, Second
  • Pregnancy
  • Organ Specificity
  • Oligonucleotide Array Sequence Analysis
  • Neurons
  • Neurogenesis
  • Neural Stem Cells
  • Immunohistochemistry
 

Citation

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MLA
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Fan, Y., Marcy, G., Lee, E. S. M., Rozen, S., Mattar, C. N. Z., Waddington, S. N., … Chan, J. K. Y. (2014). Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming. PLoS One, 9(9), e105985. https://doi.org/10.1371/journal.pone.0105985
Fan, Yiping, Guillaume Marcy, Eddy S. M. Lee, Steve Rozen, Citra N. Z. Mattar, Simon N. Waddington, Eyleen L. K. Goh, Mahesh Choolani, and Jerry K. Y. Chan. “Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming.PLoS One 9, no. 9 (2014): e105985. https://doi.org/10.1371/journal.pone.0105985.
Fan Y, Marcy G, Lee ESM, Rozen S, Mattar CNZ, Waddington SN, et al. Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming. PLoS One. 2014;9(9):e105985.
Fan, Yiping, et al. “Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming.PLoS One, vol. 9, no. 9, 2014, p. e105985. Pubmed, doi:10.1371/journal.pone.0105985.
Fan Y, Marcy G, Lee ESM, Rozen S, Mattar CNZ, Waddington SN, Goh ELK, Choolani M, Chan JKY. Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming. PLoS One. 2014;9(9):e105985.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

9

Start / End Page

e105985

Location

United States

Related Subject Headings

  • Stem Cell Transplantation
  • Spheroids, Cellular
  • Pregnancy Trimester, Second
  • Pregnancy
  • Organ Specificity
  • Oligonucleotide Array Sequence Analysis
  • Neurons
  • Neurogenesis
  • Neural Stem Cells
  • Immunohistochemistry