Microarray analysis of peroxisome proliferator-activated receptor-gamma induced changes in gene expression in macrophages.
We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) gamma in RAW264.7 macrophages. PPARgamma natural ligand 15-deoxy-Delta(12,14) prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPARgamma activation on gene expression and cognate pathways in macrophages.
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Related Subject Headings
- Transcription Factors
- Receptors, Cytoplasmic and Nuclear
- Oligonucleotide Array Sequence Analysis
- Models, Biological
- Macrophages
- Lipid Metabolism
- Gene Expression Regulation
- Gene Expression Profiling
- Cell Line
- Biochemistry & Molecular Biology
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transcription Factors
- Receptors, Cytoplasmic and Nuclear
- Oligonucleotide Array Sequence Analysis
- Models, Biological
- Macrophages
- Lipid Metabolism
- Gene Expression Regulation
- Gene Expression Profiling
- Cell Line
- Biochemistry & Molecular Biology