Skip to main content
Journal cover image

Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells: formation of intracellular lead-inclusion bodies and induction of apoptosis.

Publication ,  Journal Article
Singh, J; Pritchard, DE; Carlisle, DL; Mclean, JA; Montaser, A; Orenstein, JM; Patierno, SR
Published in: Toxicol Appl Pharmacol
December 15, 1999

Occupational exposure to certain particulate hexavalent chromium [Cr(VI)] compounds, such as lead chromate, has been associated with lung cancer and respiratory tract toxicity. We have previously shown that apoptosis is a major mode of death in cultured rodent cells treated with soluble sodium chromate and particulate lead chromate. Here we report the cellular and molecular effects of lead chromate and sodium chromate in normal human lung small airway epithelial (HSAE) cells, which may be one of the targets for Cr(VI)-induced lung cancer and respiratory tract toxicity. Phagocytosed lead chromate particles and intracellular lead-inclusion bodies (LIB) were observed by transmission electron microscopy and confirmed by X-ray analysis. HSAE cells exposed to lead chromate and sodium chromate underwent dose-dependent apoptosis. The cellular uptake and genomic interactions of both Cr and lead (Pb) were examined by inductively coupled plasma mass spectrometry (ICPMS) coupled with a novel, direct-injection high-efficiency nebulizer (DIHEN). Using this approach, we have quantitated a dose-dependent formation of Cr-DNA adducts and DNA-associated Pb in lead chromate-treated HSAE cells. The formation of LIB in normal human lung cells exposed to lead chromate indicates that ionic Pb is released from the particles and thus might contribute to the cell toxicity caused by lead chromate. Internalization and dissolution of lead chromate particles and the interaction of ionic Cr and Pb with DNA, may be components of the mechanism of lead chromate carcinogenesis. Lead chromate-induced apoptosis may be a mechanism to eliminate cells with chromium- and/or lead-damaged DNA.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Toxicol Appl Pharmacol

DOI

ISSN

0041-008X

Publication Date

December 15, 1999

Volume

161

Issue

3

Start / End Page

240 / 248

Location

United States

Related Subject Headings

  • Toxicology
  • Phagocytosis
  • Particle Size
  • Microscopy, Fluorescence
  • Lung
  • Lead
  • Inclusion Bodies
  • Humans
  • Epithelial Cells
  • Electron Probe Microanalysis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Singh, J., Pritchard, D. E., Carlisle, D. L., Mclean, J. A., Montaser, A., Orenstein, J. M., & Patierno, S. R. (1999). Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells: formation of intracellular lead-inclusion bodies and induction of apoptosis. Toxicol Appl Pharmacol, 161(3), 240–248. https://doi.org/10.1006/taap.1999.8816
Singh, J., D. E. Pritchard, D. L. Carlisle, J. A. Mclean, A. Montaser, J. M. Orenstein, and S. R. Patierno. “Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells: formation of intracellular lead-inclusion bodies and induction of apoptosis.Toxicol Appl Pharmacol 161, no. 3 (December 15, 1999): 240–48. https://doi.org/10.1006/taap.1999.8816.
Singh J, Pritchard DE, Carlisle DL, Mclean JA, Montaser A, Orenstein JM, et al. Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells: formation of intracellular lead-inclusion bodies and induction of apoptosis. Toxicol Appl Pharmacol. 1999 Dec 15;161(3):240–8.
Singh, J., et al. “Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells: formation of intracellular lead-inclusion bodies and induction of apoptosis.Toxicol Appl Pharmacol, vol. 161, no. 3, Dec. 1999, pp. 240–48. Pubmed, doi:10.1006/taap.1999.8816.
Singh J, Pritchard DE, Carlisle DL, Mclean JA, Montaser A, Orenstein JM, Patierno SR. Internalization of carcinogenic lead chromate particles by cultured normal human lung epithelial cells: formation of intracellular lead-inclusion bodies and induction of apoptosis. Toxicol Appl Pharmacol. 1999 Dec 15;161(3):240–248.
Journal cover image

Published In

Toxicol Appl Pharmacol

DOI

ISSN

0041-008X

Publication Date

December 15, 1999

Volume

161

Issue

3

Start / End Page

240 / 248

Location

United States

Related Subject Headings

  • Toxicology
  • Phagocytosis
  • Particle Size
  • Microscopy, Fluorescence
  • Lung
  • Lead
  • Inclusion Bodies
  • Humans
  • Epithelial Cells
  • Electron Probe Microanalysis