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Characterization and expression of a rice RAD23 gene.

Publication ,  Journal Article
Schultz, TF; Quatrano, RS
Published in: Plant molecular biology
June 1997

In order to identify proteins that interact with plant transcriptional complexes, we performed a two-hybrid screen in yeast using a cDNA library from embryogenic rice suspension cultures and the plant transcriptional activator viviparous-1 (vp1) as 'bait'. In this screen, we detected an interaction between VP1 and a rice homologue of the Saccharomyces cerevisiae RAD23 gene (osRAD23). The RAD23 protein is associated with the general transcriptional machinery in yeast, and is believed to play a role in the processes of nucleotide excision repair in yeast and mammalian cells. This report is the first identification of a RAD23 homolog in plants. The osRAD23 amino acid sequence shares 50-60% similarity throughout its length with RAD23 sequences from yeast, mice, and man. osRAD23 contains a characteristic ubiquitin-like domain at its N-terminus, which is similar to other RAD23 genes. Analysis of the expressed sequence tag database identifies two different classes of RAD23 genes in both Arabidopsis and rice. Southern analysis of rice genomic DNA indicated the presence of at least two RAD23-like genes. A single transcript (1.5 kb) of osRAD23 was detected in total RNA from rice embryonic tissue, while three transcripts (1.8, 1.5 and 1.0 kb) were observed in total RNA from vegetative tissues of rice.

Duke Scholars

Published In

Plant molecular biology

DOI

EISSN

1573-5028

ISSN

0167-4412

Publication Date

June 1997

Volume

34

Issue

3

Start / End Page

557 / 562

Related Subject Headings

  • Transcription, Genetic
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Saccharomyces cerevisiae
  • Plant Proteins
  • Plant Biology & Botany
  • Oryza
  • Molecular Sequence Data
  • Mice
  • Mammals
 

Citation

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Schultz, T. F., & Quatrano, R. S. (1997). Characterization and expression of a rice RAD23 gene. Plant Molecular Biology, 34(3), 557–562. https://doi.org/10.1023/a:1005870227632
Schultz, T. F., and R. S. Quatrano. “Characterization and expression of a rice RAD23 gene.Plant Molecular Biology 34, no. 3 (June 1997): 557–62. https://doi.org/10.1023/a:1005870227632.
Schultz TF, Quatrano RS. Characterization and expression of a rice RAD23 gene. Plant molecular biology. 1997 Jun;34(3):557–62.
Schultz, T. F., and R. S. Quatrano. “Characterization and expression of a rice RAD23 gene.Plant Molecular Biology, vol. 34, no. 3, June 1997, pp. 557–62. Epmc, doi:10.1023/a:1005870227632.
Schultz TF, Quatrano RS. Characterization and expression of a rice RAD23 gene. Plant molecular biology. 1997 Jun;34(3):557–562.
Journal cover image

Published In

Plant molecular biology

DOI

EISSN

1573-5028

ISSN

0167-4412

Publication Date

June 1997

Volume

34

Issue

3

Start / End Page

557 / 562

Related Subject Headings

  • Transcription, Genetic
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Saccharomyces cerevisiae
  • Plant Proteins
  • Plant Biology & Botany
  • Oryza
  • Molecular Sequence Data
  • Mice
  • Mammals