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Acute decreases in proteasome pathway activity after inhalation of fresh diesel exhaust or secondary organic aerosol.

Publication ,  Journal Article
Kipen, HM; Gandhi, S; Rich, DQ; Ohman-Strickland, P; Laumbach, R; Fan, Z-H; Chen, L; Laskin, DL; Zhang, J; Madura, K
Published in: Environmental health perspectives
May 2011

Epidemiologic studies consistently demonstrate an association between acute cardiopulmonary events and changes in air pollution; however, the mechanisms that underlie these associations are not completely understood. Oxidative stress and inflammation have been suggested to play a role in human responses to air pollution. The proteasome is an intracellular protein degradation system linked to both of these processes and may help mediate air pollution effects.In these studies, we determined whether acute experimental exposure to two different aerosols altered white blood cell (WBC) or red blood cell (RBC) proteasome activity in human subjects. One aerosol was fresh diesel exhaust (DE), and the other freshly generated secondary organic aerosol (SOA).Thirty-eight healthy subjects underwent 2-hr resting inhalation exposures to DE and separate exposures to clean air (CA); 26 subjects were exposed to DE, CA, and SOA. CA responses were subtracted from DE or SOA responses, and mixed linear models with F-tests were used to test the effect of exposure to each aerosol on WBC and RBC proteasome activity.WBC proteasome activity was reduced 8% (p = 0.04) after exposure to either DE or SOA and decreased by 11.5% (p = 0.03) when SOA was analyzed alone. RBCs showed similar 8-10% declines in proteasome activity (p = 0.05 for DE alone).Air pollution produces oxidative stress and inflammation in many experimental models, including humans. Two experimental aerosols caused rapid declines in proteasome activity in peripheral blood cells, supporting a key role for the proteasome in acute human responses to air pollution.

Duke Scholars

Published In

Environmental health perspectives

DOI

EISSN

1552-9924

ISSN

0091-6765

Publication Date

May 2011

Volume

119

Issue

5

Start / End Page

658 / 663

Related Subject Headings

  • Young Adult
  • Vehicle Emissions
  • Toxicology
  • Proteasome Endopeptidase Complex
  • Male
  • Leukocytes
  • Inhalation Exposure
  • Humans
  • Female
  • Erythrocytes
 

Citation

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MLA
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Kipen, H. M., Gandhi, S., Rich, D. Q., Ohman-Strickland, P., Laumbach, R., Fan, Z.-H., … Madura, K. (2011). Acute decreases in proteasome pathway activity after inhalation of fresh diesel exhaust or secondary organic aerosol. Environmental Health Perspectives, 119(5), 658–663. https://doi.org/10.1289/ehp.1002784
Kipen, Howard M., Sampada Gandhi, David Q. Rich, Pamela Ohman-Strickland, Robert Laumbach, Zhi-Hua Fan, Li Chen, Debra L. Laskin, Junfeng Zhang, and Kiran Madura. “Acute decreases in proteasome pathway activity after inhalation of fresh diesel exhaust or secondary organic aerosol.Environmental Health Perspectives 119, no. 5 (May 2011): 658–63. https://doi.org/10.1289/ehp.1002784.
Kipen HM, Gandhi S, Rich DQ, Ohman-Strickland P, Laumbach R, Fan Z-H, et al. Acute decreases in proteasome pathway activity after inhalation of fresh diesel exhaust or secondary organic aerosol. Environmental health perspectives. 2011 May;119(5):658–63.
Kipen, Howard M., et al. “Acute decreases in proteasome pathway activity after inhalation of fresh diesel exhaust or secondary organic aerosol.Environmental Health Perspectives, vol. 119, no. 5, May 2011, pp. 658–63. Epmc, doi:10.1289/ehp.1002784.
Kipen HM, Gandhi S, Rich DQ, Ohman-Strickland P, Laumbach R, Fan Z-H, Chen L, Laskin DL, Zhang J, Madura K. Acute decreases in proteasome pathway activity after inhalation of fresh diesel exhaust or secondary organic aerosol. Environmental health perspectives. 2011 May;119(5):658–663.

Published In

Environmental health perspectives

DOI

EISSN

1552-9924

ISSN

0091-6765

Publication Date

May 2011

Volume

119

Issue

5

Start / End Page

658 / 663

Related Subject Headings

  • Young Adult
  • Vehicle Emissions
  • Toxicology
  • Proteasome Endopeptidase Complex
  • Male
  • Leukocytes
  • Inhalation Exposure
  • Humans
  • Female
  • Erythrocytes