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Insulin and glucosamine infusions increase O-linked N-acetyl-glucosamine in skeletal muscle proteins in vivo.

Publication ,  Journal Article
Yki-Järvinen, H; Virkamäki, A; Daniels, MC; McClain, D; Gottschalk, WK
Published in: Metabolism: clinical and experimental
April 1998

O-linked N-acetylglucosamine (O-GlcNAc) is an abundant posttranslational modification of serine/threonine residues of nuclear and cytoplasmic proteins. We determined whether insulin or coinfusion of glucosamine (GlcN) with insulin alters O-GlcNAc of skeletal muscle proteins. Three groups of conscious fasted rats received 6-hour infusions of either saline (BAS), insulin 18 mU/kg.min and saline (INS), or insulin and GlcN 30 micromol/kg.min (GLCN) during maintenance of normoglycemia. At 6 hours, the concentrations of muscle UDP-GlcNAc, UDP-N-acetylgalactosamine (UDP-GalNAc), UDP-glucose (UDP-Glc), UDP-galactose (UDP-Gal), glycogen, and N and O-linked GlcNAc (galactosyltransferase labeling followed by beta elimination) were measured in freeze-clamped abdominis muscle. Insulin increased whole-body glucose uptake from 49 +/- 5 to 239 +/- 8 micromol/kg.min (P < .001) and glycogen in abdominis muscle from 138 +/- 11 to 370 +/- 26 mmol/kg dry weight (P < .001). Insulin increased the amount of cytosolic N - and O-linked GlcNAc by 56% from 362 +/- 30 to 564 +/- 45 dpm/microg protein . 100 min (P < .02), and O-GlcNAc from 221 +/- 16 to 339 +/- 27 dpm/microg . 100 min (P < .02). Glycogen content was positively correlated with the amount of total (r = .90, P < .005) and O-linked GlcNAc in insulin-infused animals. Coinfusion of GlcN with insulin increased muscle UDP-GlcNAc about fourfold (100 +/- 6 nmol/g) compared with insulin (27 +/- 1, P < .001) or saline (25 +/- 1, P < .001) infusion. GlcN also decreased glucose uptake over 6 hours by 30% to 168 +/- 8 micromol/kg . min (P < .001 for GLCN v INS) and muscle glycogen to 292 +/- 24 mmol/kg dry weight (P < .05 for GLCN v INS). Both total (635 +/- 60 dpm/microg . 100 min, P < .002) and O-linked GlcNAc (375 +/- 36 dpm/microg . 100 min, P < .002) in the cytosol were significantly higher in GLCN rats (635 +/- 60 dpm/microg) versus BAS rats (P < .002). As in INS rats, muscle glycogen and O-GlcNAc were positively correlated in GLCN rats (r = .54, P < .05). Variation in total and O-linked GlcNAc in GLCN rats was due both to GlcN (P < .02) and to variation in the glycogen content (P < .005).

Duke Scholars

Published In

Metabolism: clinical and experimental

DOI

EISSN

1532-8600

ISSN

0026-0495

Publication Date

April 1998

Volume

47

Issue

4

Start / End Page

449 / 455

Related Subject Headings

  • Uridine Diphosphate Galactose
  • Rats, Wistar
  • Rats
  • Muscle, Skeletal
  • Muscle Proteins
  • Male
  • Insulin
  • Infusions, Parenteral
  • Infusions, Intravenous
  • Glycolysis
 

Citation

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Yki-Järvinen, H., Virkamäki, A., Daniels, M. C., McClain, D., & Gottschalk, W. K. (1998). Insulin and glucosamine infusions increase O-linked N-acetyl-glucosamine in skeletal muscle proteins in vivo. Metabolism: Clinical and Experimental, 47(4), 449–455. https://doi.org/10.1016/s0026-0495(98)90058-0
Yki-Järvinen, H., A. Virkamäki, M. C. Daniels, D. McClain, and W. K. Gottschalk. “Insulin and glucosamine infusions increase O-linked N-acetyl-glucosamine in skeletal muscle proteins in vivo.Metabolism: Clinical and Experimental 47, no. 4 (April 1998): 449–55. https://doi.org/10.1016/s0026-0495(98)90058-0.
Yki-Järvinen H, Virkamäki A, Daniels MC, McClain D, Gottschalk WK. Insulin and glucosamine infusions increase O-linked N-acetyl-glucosamine in skeletal muscle proteins in vivo. Metabolism: clinical and experimental. 1998 Apr;47(4):449–55.
Yki-Järvinen, H., et al. “Insulin and glucosamine infusions increase O-linked N-acetyl-glucosamine in skeletal muscle proteins in vivo.Metabolism: Clinical and Experimental, vol. 47, no. 4, Apr. 1998, pp. 449–55. Epmc, doi:10.1016/s0026-0495(98)90058-0.
Yki-Järvinen H, Virkamäki A, Daniels MC, McClain D, Gottschalk WK. Insulin and glucosamine infusions increase O-linked N-acetyl-glucosamine in skeletal muscle proteins in vivo. Metabolism: clinical and experimental. 1998 Apr;47(4):449–455.
Journal cover image

Published In

Metabolism: clinical and experimental

DOI

EISSN

1532-8600

ISSN

0026-0495

Publication Date

April 1998

Volume

47

Issue

4

Start / End Page

449 / 455

Related Subject Headings

  • Uridine Diphosphate Galactose
  • Rats, Wistar
  • Rats
  • Muscle, Skeletal
  • Muscle Proteins
  • Male
  • Insulin
  • Infusions, Parenteral
  • Infusions, Intravenous
  • Glycolysis