Scholars@Duke publication: Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials.

Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials.

Publication , Journal Article

Thompson, JW; Lieberman, RA; Jorgenson, JW

Published in: J Chromatogr A

November 6, 2009

Hydrodynamic chromatography (HDC) was used as a size classification and purification method for porous bridged ethyl hybrid (BEH) packing materials (particles) in the micron to sub-micron range. Using packed column HDC, a batch of particles with size 0.76+/-0.26microm was fractionated to yield classified material of 1.05+/-0.16microm, reducing the relative standard deviation from 33% to 15%. Subsequent chromatographic evaluation of this packing material showed significant improvement in column performance and decrease in flow resistance over the unclassified material. Comparing a column packed with the classified versus non-classified material, the effective flow resistance of the two columns was decreased by 58% and the minimum HETP for the packing material was improved from 4 to 2.5microm.

Duke Scholars

Author J. Will Thompson Pharmacology & Cancer Biology

Published In

J Chromatogr A

DOI

10.1016/j.chroma.2009.08.088

EISSN

1873-3778

Publication Date

November 6, 2009

Volume

1216

Issue

Start / End Page

7732 / 7738

Location

Netherlands

Related Subject Headings

Resins, Synthetic
Particle Size
Chromatography, Liquid
Analytical Chemistry
40 Engineering
34 Chemical sciences
10 Technology
09 Engineering
03 Chemical Sciences

Citation

APA

Chicago

ICMJE

MLA

NLM

Thompson, J. W., Lieberman, R. A., & Jorgenson, J. W. (2009). Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials. J Chromatogr A, 1216(45), 7732–7738. https://doi.org/10.1016/j.chroma.2009.08.088

Thompson, J Will, Rachel A. Lieberman, and James W. Jorgenson. “Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials.” J Chromatogr A 1216, no. 45 (November 6, 2009): 7732–38. https://doi.org/10.1016/j.chroma.2009.08.088.

Thompson JW, Lieberman RA, Jorgenson JW. Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials. J Chromatogr A. 2009 Nov 6;1216(45):7732–8.

Thompson, J. Will, et al. “Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials.” J Chromatogr A, vol. 1216, no. 45, Nov. 2009, pp. 7732–38. Pubmed, doi:10.1016/j.chroma.2009.08.088.

Thompson JW, Lieberman RA, Jorgenson JW. Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials. J Chromatogr A. 2009 Nov 6;1216(45):7732–7738.