Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials.
Hydrodynamic chromatography (HDC) was used as a size classification and purification method for porous bridged ethyl hybrid (BEH) packing materials (particles) in the micron to sub-micron range. Using packed column HDC, a batch of particles with size 0.76+/-0.26microm was fractionated to yield classified material of 1.05+/-0.16microm, reducing the relative standard deviation from 33% to 15%. Subsequent chromatographic evaluation of this packing material showed significant improvement in column performance and decrease in flow resistance over the unclassified material. Comparing a column packed with the classified versus non-classified material, the effective flow resistance of the two columns was decreased by 58% and the minimum HETP for the packing material was improved from 4 to 2.5microm.
Duke Scholars
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Resins, Synthetic
- Particle Size
- Chromatography, Liquid
- Analytical Chemistry
- 40 Engineering
- 34 Chemical sciences
- 10 Technology
- 09 Engineering
- 03 Chemical Sciences
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Resins, Synthetic
- Particle Size
- Chromatography, Liquid
- Analytical Chemistry
- 40 Engineering
- 34 Chemical sciences
- 10 Technology
- 09 Engineering
- 03 Chemical Sciences