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Identification of JWA as a novel functional gene responsive to environmental oxidative stress induced by benzo[a]pyrene and hydrogen peroxide.

Publication ,  Journal Article
Chen, R; Qiu, W; Liu, Z; Cao, X; Zhu, T; Li, A; Wei, Q; Zhou, J
Published in: Free Radic Biol Med
June 1, 2007

Oxidative stress has been implicated as one of the primary mechanisms inducing DNA damage and believed to mediate aging and progression of numerous age-related diseases, including cancer. JWA, a gene previously described to mediate differentiation of leukemic cells, is also involved in cellular responses to environmental exposures linked to heat shock and chemical-mediated oxidative stresses. However, the precise pathways and mechanisms underlying these phenomena remain to be resolved. Our studies demonstrated that H(2)O(2) is the primary oxidative product responsible for benzo[a]pyrene (B[a]P)-induced JWA expression, and knockdown of JWA elevates H(2)O(2) (100 microM)- and B[a]P (100 microM)-induced DNA damage. In oxidative stress cell culture models, JWA was upregulated. JWA expression regulated a parallel rise in the base excision repair protein XRCC1 but a reduction in PARP1 in response to H(2)O(2)-induced DNA damage. Furthermore, we found that both H(2)O(2) and B[a]P exposure activated nuclear transcription factor I (NFI) in NIH-3T3 cells, which specifically bound to the CCAAT element in the JWA proximal promoter (-58/-28 bp) and thereby induced JWA expression. Consistently siRNA mediated a knockdown of NFI, which prevented JWA induction. These findings indicate that JWA may serve as a novel environmental stress sensor to protect cells against reactive oxygen species-associated DNA damage.

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Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

June 1, 2007

Volume

42

Issue

11

Start / End Page

1704 / 1714

Location

United States

Related Subject Headings

  • Promoter Regions, Genetic
  • Oxidative Stress
  • NIH 3T3 Cells
  • NFI Transcription Factors
  • Molecular Sequence Data
  • Mice
  • Membrane Transport Proteins
  • Hydrogen Peroxide
  • Humans
  • Heat-Shock Proteins
 

Citation

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Chen, R., Qiu, W., Liu, Z., Cao, X., Zhu, T., Li, A., … Zhou, J. (2007). Identification of JWA as a novel functional gene responsive to environmental oxidative stress induced by benzo[a]pyrene and hydrogen peroxide. Free Radic Biol Med, 42(11), 1704–1714. https://doi.org/10.1016/j.freeradbiomed.2007.02.018
Chen, Rui, Wen Qiu, Zulong Liu, Xingjiang Cao, Ting Zhu, Aiping Li, Qingyi Wei, and Jianwei Zhou. “Identification of JWA as a novel functional gene responsive to environmental oxidative stress induced by benzo[a]pyrene and hydrogen peroxide.Free Radic Biol Med 42, no. 11 (June 1, 2007): 1704–14. https://doi.org/10.1016/j.freeradbiomed.2007.02.018.
Chen R, Qiu W, Liu Z, Cao X, Zhu T, Li A, et al. Identification of JWA as a novel functional gene responsive to environmental oxidative stress induced by benzo[a]pyrene and hydrogen peroxide. Free Radic Biol Med. 2007 Jun 1;42(11):1704–14.
Chen, Rui, et al. “Identification of JWA as a novel functional gene responsive to environmental oxidative stress induced by benzo[a]pyrene and hydrogen peroxide.Free Radic Biol Med, vol. 42, no. 11, June 2007, pp. 1704–14. Pubmed, doi:10.1016/j.freeradbiomed.2007.02.018.
Chen R, Qiu W, Liu Z, Cao X, Zhu T, Li A, Wei Q, Zhou J. Identification of JWA as a novel functional gene responsive to environmental oxidative stress induced by benzo[a]pyrene and hydrogen peroxide. Free Radic Biol Med. 2007 Jun 1;42(11):1704–1714.
Journal cover image

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

June 1, 2007

Volume

42

Issue

11

Start / End Page

1704 / 1714

Location

United States

Related Subject Headings

  • Promoter Regions, Genetic
  • Oxidative Stress
  • NIH 3T3 Cells
  • NFI Transcription Factors
  • Molecular Sequence Data
  • Mice
  • Membrane Transport Proteins
  • Hydrogen Peroxide
  • Humans
  • Heat-Shock Proteins