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Risk assessment for developing gliomas: a comparison of two cytogenetic approaches.

Publication ,  Journal Article
El-Zein, R; Bondy, ML; Wang, LE; de Andrade, M; Sigurdson, AJ; Bruner, JM; Kyritsis, AP; Levin, VA; Wei, Q
Published in: Mutat Res
January 25, 2001

Chromosome instability (CIN) measured as chromosome aberrations has long been suggested as a cancer susceptibility biomarker. Conventional cytogenetic end-points are now being improved by combining molecular methods, which increases the sensitivity, specificity, and precision of the assay. In this study we examined both spontaneous and gamma-ray induced CIN in lymphocyte cultures from 51 previously untreated glioma patients and 51 age-, sex- and ethnicity-matched controls. CIN was assessed using two parallel methods: (1) the mutagen sensitivity (MS) assay and (2) the multicolor fluorescence in situ hybridization (FISH) assay. The frequency of spontaneous breaks was significantly higher in glioma patients (mean+/-S.D., 2.12+/-1.07) than in controls (1.24+/-0.86, P<0.001) when using the FISH assay but not the MS assay (0.019+/-0.02 and 0.019+/-0.01, respectively; P=0.915). Similarly, the frequency of induced chromatid breaks was significantly higher using the FISH assay (3.39+/-1.72) but not the MS assay (0.42+/-0.16) in the patients versus controls (2.08+/-1.18 and 0.37+/-0.15, respectively; P<0.001 and P=0.10, respectively). By using the median number of breaks in the controls as the cutoff value, we observed an odds ratio (ORs) of 5.13 (95% CI=2.23-12.1) for spontaneous and 4.86 (95% CI=2.08-11.4) for induced CIN using the FISH assay, whereas the ORs were 1.32 (95% CI=0.49-3.58) and 1.28 (95% CI=0.59-2.80) for spontaneous and induced CIN using the MS assay. There was also a significant increase in the frequency of hyperdiploid cells in the glioma cases which could only be detected using the FISH assay (OR=4.0, 95% CL=0.9-17.0). By combining both methods an estimated risk of 7.0 (95% CI=1.7-25.6) was observed. There was no correlation between the breaks detected by the two methods suggesting that each method is a measure of a different event. The results indicate that using the multicolor FISH assay for detection of CIN in peripheral blood lymphocytes in glioma patients is a more useful marker for risk assessment.

Duke Scholars

Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

January 25, 2001

Volume

490

Issue

1

Start / End Page

35 / 44

Location

Netherlands

Related Subject Headings

  • Oncology & Carcinogenesis
  • Middle Aged
  • Micronucleus Tests
  • Male
  • In Situ Hybridization, Fluorescence
  • Humans
  • Glioma
  • Genetic Testing
  • Female
  • Chromosome Aberrations
 

Citation

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El-Zein, R., Bondy, M. L., Wang, L. E., de Andrade, M., Sigurdson, A. J., Bruner, J. M., … Wei, Q. (2001). Risk assessment for developing gliomas: a comparison of two cytogenetic approaches. Mutat Res, 490(1), 35–44. https://doi.org/10.1016/s1383-5718(00)00154-6
El-Zein, R., M. L. Bondy, L. E. Wang, M. de Andrade, A. J. Sigurdson, J. M. Bruner, A. P. Kyritsis, V. A. Levin, and Q. Wei. “Risk assessment for developing gliomas: a comparison of two cytogenetic approaches.Mutat Res 490, no. 1 (January 25, 2001): 35–44. https://doi.org/10.1016/s1383-5718(00)00154-6.
El-Zein R, Bondy ML, Wang LE, de Andrade M, Sigurdson AJ, Bruner JM, et al. Risk assessment for developing gliomas: a comparison of two cytogenetic approaches. Mutat Res. 2001 Jan 25;490(1):35–44.
El-Zein, R., et al. “Risk assessment for developing gliomas: a comparison of two cytogenetic approaches.Mutat Res, vol. 490, no. 1, Jan. 2001, pp. 35–44. Pubmed, doi:10.1016/s1383-5718(00)00154-6.
El-Zein R, Bondy ML, Wang LE, de Andrade M, Sigurdson AJ, Bruner JM, Kyritsis AP, Levin VA, Wei Q. Risk assessment for developing gliomas: a comparison of two cytogenetic approaches. Mutat Res. 2001 Jan 25;490(1):35–44.
Journal cover image

Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

January 25, 2001

Volume

490

Issue

1

Start / End Page

35 / 44

Location

Netherlands

Related Subject Headings

  • Oncology & Carcinogenesis
  • Middle Aged
  • Micronucleus Tests
  • Male
  • In Situ Hybridization, Fluorescence
  • Humans
  • Glioma
  • Genetic Testing
  • Female
  • Chromosome Aberrations