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Expression in normal human tissues of five nucleotide excision repair genes measured simultaneously by multiplex reverse transcription-polymerase chain reaction.

Publication ,  Journal Article
Cheng, L; Guan, Y; Li, L; Legerski, RJ; Einspahr, J; Bangert, J; Alberts, DS; Wei, Q
Published in: Cancer Epidemiol Biomarkers Prev
September 1999

DNA repair is central to the integrity of the human genome. Reduced DNA repair capacity has been linked to genetic susceptibility to cancer. An adequate expression level of DNA repair genes is essential for normal DNA repair activities. Although there is tissue specificity in the expression, searching for a surrogate tissue is needed for molecular epidemiological studies. In this study, the relative expression levels of five selected human nucleotide excision repair (NER) genes (ERCC1, XPB/ERCC3, XPG/ERCC5, CSB/ERCC6, and XPC) in 20 different types of human normal tissue were simultaneously measured by a new multiplex reverse transcription (RT)-PCR assay using the expression level of the beta-actin gene as an internal control. Transcripts of each of the five NER genes were detectable, but the levels varied in these normal tissues. Both mitogen (phytohemagglutinin)-stimulated and unstimulated human peripheral lymphocytes showed similar expression patterns for the five NER genes. In general, the expression levels of stimulated lymphocytes were also similar to most of the rapidly proliferating tissues, such as the skin, breast, intestine, liver, testis, ovary, placenta, or prostate, but was relatively higher than that of the slowly proliferating or nonproliferating tissues such as adipose, brain, hippocampus, muscle, spleen, or lung. The data suggested that although the five NER genes were expressed at different levels in the normal tissues examined, PHA-stimulated peripheral lymphocytes may be used as a surrogate tissue for estimating expression levels of these genes in proliferating tissues. This new multiplex RT-PCR assay may help detect aberrant expression of these NER genes in both normal and tumor tissues.

Duke Scholars

Published In

Cancer Epidemiol Biomarkers Prev

ISSN

1055-9965

Publication Date

September 1999

Volume

8

Issue

9

Start / End Page

801 / 807

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Reference Values
  • Humans
  • Gene Expression
  • Epidemiology
  • DNA Repair
  • DNA Primers
  • DNA
  • Culture Techniques
  • Cells, Cultured
 

Citation

APA
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ICMJE
MLA
NLM
Cheng, L., Guan, Y., Li, L., Legerski, R. J., Einspahr, J., Bangert, J., … Wei, Q. (1999). Expression in normal human tissues of five nucleotide excision repair genes measured simultaneously by multiplex reverse transcription-polymerase chain reaction. Cancer Epidemiol Biomarkers Prev, 8(9), 801–807.
Cheng, L., Y. Guan, L. Li, R. J. Legerski, J. Einspahr, J. Bangert, D. S. Alberts, and Q. Wei. “Expression in normal human tissues of five nucleotide excision repair genes measured simultaneously by multiplex reverse transcription-polymerase chain reaction.Cancer Epidemiol Biomarkers Prev 8, no. 9 (September 1999): 801–7.
Cheng L, Guan Y, Li L, Legerski RJ, Einspahr J, Bangert J, et al. Expression in normal human tissues of five nucleotide excision repair genes measured simultaneously by multiplex reverse transcription-polymerase chain reaction. Cancer Epidemiol Biomarkers Prev. 1999 Sep;8(9):801–7.
Cheng L, Guan Y, Li L, Legerski RJ, Einspahr J, Bangert J, Alberts DS, Wei Q. Expression in normal human tissues of five nucleotide excision repair genes measured simultaneously by multiplex reverse transcription-polymerase chain reaction. Cancer Epidemiol Biomarkers Prev. 1999 Sep;8(9):801–807.

Published In

Cancer Epidemiol Biomarkers Prev

ISSN

1055-9965

Publication Date

September 1999

Volume

8

Issue

9

Start / End Page

801 / 807

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Reference Values
  • Humans
  • Gene Expression
  • Epidemiology
  • DNA Repair
  • DNA Primers
  • DNA
  • Culture Techniques
  • Cells, Cultured