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Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells.

Publication ,  Journal Article
Seymour, K; Han, X; Sadowitz, B; Maier, KG; Gahtan, V
Published in: Am J Surg
November 2010

BACKGROUND: Neointimal hyperplasia involves the migration of medial vascular smooth muscle cells (VSMCs) in response to arterial injury. Thrombospondin-1 (TSP1), platelet-derived growth factor (PDGF), and fibronectin (Fn) induce VSMC migration. Nitric oxide (NO) limits VSMC migration. The hypothesis of this study is that NO would dose dependently inhibit TSP1-induced, PDGF-induced, and Fn-induced VSMC chemotaxis. METHODS: VSMCs were pretreated with serum free media or the NO donors diethylenetriamine NONOate or S-nitroso-N-acetyl-D,L-penicillamine. Chemotaxis to TSP1, PDGF, or Fn was determined. Analysis of variance with post hoc testing was done. P values < .05 were considered significant. RESULTS: PDGF, TSP1, and Fn induced VSMC chemotaxis. NO donors inhibited chemotaxis of VSMCs to PDGF in a concentration-dependent manner. NO donors had a variable effect on TSP1-induced chemotaxis. NO donors did not inhibit Fn-induced chemotaxis. CONCLUSION: The complex interactions of these proteins in vivo will need to be considered when developing NO-dependent therapies for neointimal hyperplasia.

Duke Scholars

Published In

Am J Surg

DOI

EISSN

1879-1883

Publication Date

November 2010

Volume

200

Issue

5

Start / End Page

615 / 619

Location

United States

Related Subject Headings

  • Thrombospondin 1
  • Surgery
  • Signal Transduction
  • Platelet-Derived Growth Factor
  • Nitric Oxide
  • Muscle, Smooth, Vascular
  • Humans
  • Fibronectins
  • Cells, Cultured
  • Cell Proliferation
 

Citation

APA
Chicago
ICMJE
MLA
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Seymour, K., Han, X., Sadowitz, B., Maier, K. G., & Gahtan, V. (2010). Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells. Am J Surg, 200(5), 615–619. https://doi.org/10.1016/j.amjsurg.2010.07.018
Seymour, Keri, Xuan Han, Benjamin Sadowitz, Kristopher G. Maier, and Vivian Gahtan. “Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells.Am J Surg 200, no. 5 (November 2010): 615–19. https://doi.org/10.1016/j.amjsurg.2010.07.018.
Seymour, Keri, et al. “Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells.Am J Surg, vol. 200, no. 5, Nov. 2010, pp. 615–19. Pubmed, doi:10.1016/j.amjsurg.2010.07.018.
Seymour K, Han X, Sadowitz B, Maier KG, Gahtan V. Differential effect of nitric oxide on thrombospondin-1-, PDGF- and fibronectin-induced migration of vascular smooth muscle cells. Am J Surg. 2010 Nov;200(5):615–619.
Journal cover image

Published In

Am J Surg

DOI

EISSN

1879-1883

Publication Date

November 2010

Volume

200

Issue

5

Start / End Page

615 / 619

Location

United States

Related Subject Headings

  • Thrombospondin 1
  • Surgery
  • Signal Transduction
  • Platelet-Derived Growth Factor
  • Nitric Oxide
  • Muscle, Smooth, Vascular
  • Humans
  • Fibronectins
  • Cells, Cultured
  • Cell Proliferation