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In vivo assessment of retinal neuronal layers in multiple sclerosis with manual and automated optical coherence tomography segmentation techniques.

Publication ,  Journal Article
Seigo, MA; Sotirchos, ES; Newsome, S; Babiarz, A; Eckstein, C; Ford, E; Oakley, JD; Syc, SB; Frohman, TC; Ratchford, JN; Balcer, LJ; Saidha, S ...
Published in: J Neurol
October 2012

Macular optical coherence tomography (OCT) segmentation, enabling quantification of retinal axonal and neuronal subpopulations, may help elucidate the neuroretinal pathobiology of multiple sclerosis (MS). This study aimed to determine the agreement, reproducibility, and visual correlations of retinal layer thicknesses measured by different OCT segmentation techniques, on two spectral-domain OCT devices. Macular scans of 52 MS patients and 30 healthy controls from Spectralis OCT and Cirrus HD-OCT were segmented using fully manual (Spectralis), computer-aided manual (Spectralis and Cirrus), and fully automated (Cirrus) segmentation techniques. Letter acuity was recorded. Bland-Altman analyses revealed low mean differences across OCT segmentation techniques on both devices for ganglion cell + inner plexiform layers (GCIP; 0.76-2.43 μm), inner nuclear + outer plexiform layers (INL + OPL; 0.36-1.04 μm), and outer nuclear layers including photoreceptor segment (ONL + PR; 1.29-3.52 μm) thicknesses. Limits of agreement for GCIP and ONL + PR thicknesses were narrow. Results of fully manual and computer-aided manual segmentation were comparable to those of fully automated segmentation. MS patients demonstrated macular RNFL, GCIP, and ONL + PR thinning compared to healthy controls across OCT segmentation techniques, irrespective of device (p < 0.03 for all). Low-contrast letter acuity in MS correlated significantly and more strongly with GCIP than peripapillary RNFL thicknesses, regardless of the segmentation method or device. GCIP and ONL + PR thicknesses, measured by different OCT devices and segmentation techniques, are reproducible and agree at the individual and cohort levels. GCIP thinning in MS correlates with visual dysfunction. Significant ONL + PR thinning, detectable across OCT segmentation techniques and devices, strongly supports ONL pathology in MS. Fully automated, fully manual and computer-assisted manual OCT segmentation techniques compare closely, highlighting the utility of accurate and time-efficient automated segmentation outcomes in MS clinical trials.

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Published In

J Neurol

DOI

EISSN

1432-1459

Publication Date

October 2012

Volume

259

Issue

10

Start / End Page

2119 / 2130

Location

Germany

Related Subject Headings

  • Young Adult
  • Tomography, Optical Coherence
  • Retinal Neurons
  • Neurology & Neurosurgery
  • Multiple Sclerosis
  • Middle Aged
  • Male
  • Image Interpretation, Computer-Assisted
  • Humans
  • Female
 

Citation

APA
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Seigo, M. A., Sotirchos, E. S., Newsome, S., Babiarz, A., Eckstein, C., Ford, E., … Saidha, S. (2012). In vivo assessment of retinal neuronal layers in multiple sclerosis with manual and automated optical coherence tomography segmentation techniques. J Neurol, 259(10), 2119–2130. https://doi.org/10.1007/s00415-012-6466-x
Seigo, Michaela A., Elias S. Sotirchos, Scott Newsome, Aleksandra Babiarz, Christopher Eckstein, E’tona Ford, Jonathan D. Oakley, et al. “In vivo assessment of retinal neuronal layers in multiple sclerosis with manual and automated optical coherence tomography segmentation techniques.J Neurol 259, no. 10 (October 2012): 2119–30. https://doi.org/10.1007/s00415-012-6466-x.
Seigo MA, Sotirchos ES, Newsome S, Babiarz A, Eckstein C, Ford E, et al. In vivo assessment of retinal neuronal layers in multiple sclerosis with manual and automated optical coherence tomography segmentation techniques. J Neurol. 2012 Oct;259(10):2119–30.
Seigo, Michaela A., et al. “In vivo assessment of retinal neuronal layers in multiple sclerosis with manual and automated optical coherence tomography segmentation techniques.J Neurol, vol. 259, no. 10, Oct. 2012, pp. 2119–30. Pubmed, doi:10.1007/s00415-012-6466-x.
Seigo MA, Sotirchos ES, Newsome S, Babiarz A, Eckstein C, Ford E, Oakley JD, Syc SB, Frohman TC, Ratchford JN, Balcer LJ, Frohman EM, Calabresi PA, Saidha S. In vivo assessment of retinal neuronal layers in multiple sclerosis with manual and automated optical coherence tomography segmentation techniques. J Neurol. 2012 Oct;259(10):2119–2130.
Journal cover image

Published In

J Neurol

DOI

EISSN

1432-1459

Publication Date

October 2012

Volume

259

Issue

10

Start / End Page

2119 / 2130

Location

Germany

Related Subject Headings

  • Young Adult
  • Tomography, Optical Coherence
  • Retinal Neurons
  • Neurology & Neurosurgery
  • Multiple Sclerosis
  • Middle Aged
  • Male
  • Image Interpretation, Computer-Assisted
  • Humans
  • Female