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Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis.

Publication ,  Journal Article
Strauss, BH; Chisholm, RJ; Keeley, FW; Gotlieb, AI; Logan, RA; Armstrong, PW
Published in: Circ Res
October 1994

Remodeling of the vessel wall after balloon angioplasty injury is incompletely understood, and in particular, the role of extracellular matrix synthesis in restenosis has received little attention. The objective of the present study was to determine the sequence of changes in collagen, elastin, and proteoglycan synthesis and content after balloon injury and to relate these changes to growth of the intimal lesions and extent of cell proliferation. In a double-injury non-cholesterol-fed model, right iliac arterial lesions in 43 rabbits were treated with balloon angioplasty, and the rabbits were killed at five time points ranging from immediate to 12 weeks. Vessel wall collagen and elastin content and synthesis were measured after incubation with 14C-proline and separation with a cyanogen bromide extraction procedure. Sulfated glycosaminoglycan synthesis was measured after incubation with [35S]sulfate, papain digestion, and ethanol precipitation. Continuous in vivo infusion of bromodeoxyuridine (96 hours) was used to assess cell proliferation. The intimal area significantly increased from 0.27 +/- 0.08 to 0.73 +/- 0.11 mm2 between 0 and 12 weeks. Intimal and medial cell proliferation were modest and peaked at 1 week (labeling indexes of 4.8% and 3.0%, respectively) and then markedly declined by 2 weeks. Significant increases in collagen, elastin, and proteoglycan synthesis, up to 4 to 10 times above control nondamaged contralateral iliac arteries, were noted at 1, 2, and 4 weeks. These increases in synthesis were accompanied by significant increases in collagen and elastin content (by approximately 35%) that coincided with the temporal increase in cross-sectional area.(ABSTRACT TRUNCATED AT 250 WORDS)

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Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

October 1994

Volume

75

Issue

4

Start / End Page

650 / 658

Location

United States

Related Subject Headings

  • Tunica Media
  • Tunica Intima
  • Time Factors
  • Recurrence
  • Rabbits
  • Male
  • Iliac Artery
  • Histological Techniques
  • Glycosaminoglycans
  • Extracellular Matrix
 

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Strauss, B. H., Chisholm, R. J., Keeley, F. W., Gotlieb, A. I., Logan, R. A., & Armstrong, P. W. (1994). Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis. Circ Res, 75(4), 650–658. https://doi.org/10.1161/01.res.75.4.650
Strauss, B. H., R. J. Chisholm, F. W. Keeley, A. I. Gotlieb, R. A. Logan, and P. W. Armstrong. “Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis.Circ Res 75, no. 4 (October 1994): 650–58. https://doi.org/10.1161/01.res.75.4.650.
Strauss BH, Chisholm RJ, Keeley FW, Gotlieb AI, Logan RA, Armstrong PW. Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis. Circ Res. 1994 Oct;75(4):650–8.
Strauss, B. H., et al. “Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis.Circ Res, vol. 75, no. 4, Oct. 1994, pp. 650–58. Pubmed, doi:10.1161/01.res.75.4.650.
Strauss BH, Chisholm RJ, Keeley FW, Gotlieb AI, Logan RA, Armstrong PW. Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis. Circ Res. 1994 Oct;75(4):650–658.

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

October 1994

Volume

75

Issue

4

Start / End Page

650 / 658

Location

United States

Related Subject Headings

  • Tunica Media
  • Tunica Intima
  • Time Factors
  • Recurrence
  • Rabbits
  • Male
  • Iliac Artery
  • Histological Techniques
  • Glycosaminoglycans
  • Extracellular Matrix