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N-acetylcysteine does not modify nitroglycerin-induced tolerance in canine vascular rings.

Publication ,  Journal Article
Abdollah, A; Moffat, JA; Armstrong, PW
Published in: J Cardiovasc Pharmacol
April 1987

The mechanism whereby nitroglycerin initiates relaxation in vascular smooth muscle remains unclear. One hypothesis states that nitroglycerin oxidizes critical sulfhydryl groups in smooth muscle to initiate relaxation, and that repeated exposure to nitroglycerin results in tolerance. In the current study, N-acetylcysteine, a sulfhydryl-reducing agent, was used to explore the sulfhydryl hypothesis by assessing whether or not tolerance to nitroglycerin was reversed by N-acetylcysteine in canine dorsal pedal artery rings. Two nitroglycerin dose-response curves were performed (n = 18)--one before (1st dose-response curve, from 10(-9) to 1.1 X 10(-5) M nitroglycerin) and one after (2nd dose-response curve, from 10(-9) to 5 X 10(-7) M nitroglycerin) incubation with 10(-5) M nitroglycerin for 105 min. At 5 X 10(-7) M nitroglycerin there was 50.7 +/- 10.0% relaxation during the first dose-response curve. During the second dose-response curve, tolerance to nitroglycerin was evident, as demonstrated by a 6.8 +/- 4.8% relaxation (p less than 0.001) at 5 X 10(-7) M nitroglycerin. A 10-min treatment with 10(-3) M N-acetylcysteine (n = 10) during the second nitroglycerin dose-response curve was performed after the 5 X 10(-7) M concentration of nitroglycerin; the second dose-response curve was then completed up to 1.1 X 10(-5) M nitroglycerin. The dose of 10(-3) M N-acetylcysteine was chosen since higher concentrations (i.e., 1.3 X 10(-2) and 1.2 X 10(-1) M N-acetylcysteine) produced 20.3 +/- 8.4 and 43.6 +/- 11.6% relaxation in vascular rings (n = 5).(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

J Cardiovasc Pharmacol

DOI

ISSN

0160-2446

Publication Date

April 1987

Volume

9

Issue

4

Start / End Page

445 / 450

Location

United States

Related Subject Headings

  • Nitroglycerin
  • Muscle, Smooth, Vascular
  • Muscle Relaxation
  • Muscle Contraction
  • In Vitro Techniques
  • Drug Tolerance
  • Drug Interactions
  • Dogs
  • Cardiovascular System & Hematology
  • Animals
 

Citation

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Abdollah, A., Moffat, J. A., & Armstrong, P. W. (1987). N-acetylcysteine does not modify nitroglycerin-induced tolerance in canine vascular rings. J Cardiovasc Pharmacol, 9(4), 445–450. https://doi.org/10.1097/00005344-198704000-00009
Abdollah, A., J. A. Moffat, and P. W. Armstrong. “N-acetylcysteine does not modify nitroglycerin-induced tolerance in canine vascular rings.J Cardiovasc Pharmacol 9, no. 4 (April 1987): 445–50. https://doi.org/10.1097/00005344-198704000-00009.
Abdollah A, Moffat JA, Armstrong PW. N-acetylcysteine does not modify nitroglycerin-induced tolerance in canine vascular rings. J Cardiovasc Pharmacol. 1987 Apr;9(4):445–50.
Abdollah, A., et al. “N-acetylcysteine does not modify nitroglycerin-induced tolerance in canine vascular rings.J Cardiovasc Pharmacol, vol. 9, no. 4, Apr. 1987, pp. 445–50. Pubmed, doi:10.1097/00005344-198704000-00009.
Abdollah A, Moffat JA, Armstrong PW. N-acetylcysteine does not modify nitroglycerin-induced tolerance in canine vascular rings. J Cardiovasc Pharmacol. 1987 Apr;9(4):445–450.

Published In

J Cardiovasc Pharmacol

DOI

ISSN

0160-2446

Publication Date

April 1987

Volume

9

Issue

4

Start / End Page

445 / 450

Location

United States

Related Subject Headings

  • Nitroglycerin
  • Muscle, Smooth, Vascular
  • Muscle Relaxation
  • Muscle Contraction
  • In Vitro Techniques
  • Drug Tolerance
  • Drug Interactions
  • Dogs
  • Cardiovascular System & Hematology
  • Animals