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NgBR is essential for endothelial cell glycosylation and vascular development.

Publication ,  Journal Article
Park, EJ; Grabińska, KA; Guan, Z; Sessa, WC
Published in: EMBO Rep
February 2016

NgBR is a transmembrane protein identified as a Nogo-B-interacting protein and recently has been shown to be a subunit required for cis-prenyltransferase (cisPTase) activity. To investigate the integrated role of NgBR in vascular development, we have characterized endothelial-specific NgBR knockout embryos. Here, we show that endothelial-specific NgBR knockout results in embryonic lethality due to vascular development defects in yolk sac and embryo proper. Loss of NgBR in endothelial cells reduces proliferation and promotes apoptosis of the cells largely through defects in the glycosylation of key endothelial proteins including VEGFR2, VE-cadherin, and CD31, and defective glycosylation can be rescued by treatment with the end product of cisPTase activity, dolichol phosphate. Moreover, NgBR functions in endothelial cells during embryogenesis are Nogo-B independent. These data uniquely show the importance of NgBR and protein glycosylation during vascular development.

Duke Scholars

Published In

EMBO Rep

DOI

EISSN

1469-3178

Publication Date

February 2016

Volume

17

Issue

2

Start / End Page

167 / 177

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Receptors, Cell Surface
  • Protein Processing, Post-Translational
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Mice
  • Glycosylation
  • Endothelium, Vascular
  • Developmental Biology
  • Cells, Cultured
  • Cell Proliferation
 

Citation

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Park, E. J., Grabińska, K. A., Guan, Z., & Sessa, W. C. (2016). NgBR is essential for endothelial cell glycosylation and vascular development. EMBO Rep, 17(2), 167–177. https://doi.org/10.15252/embr.201540789
Park, Eon Joo, Kariona A. Grabińska, Ziqiang Guan, and William C. Sessa. “NgBR is essential for endothelial cell glycosylation and vascular development.EMBO Rep 17, no. 2 (February 2016): 167–77. https://doi.org/10.15252/embr.201540789.
Park EJ, Grabińska KA, Guan Z, Sessa WC. NgBR is essential for endothelial cell glycosylation and vascular development. EMBO Rep. 2016 Feb;17(2):167–77.
Park, Eon Joo, et al. “NgBR is essential for endothelial cell glycosylation and vascular development.EMBO Rep, vol. 17, no. 2, Feb. 2016, pp. 167–77. Pubmed, doi:10.15252/embr.201540789.
Park EJ, Grabińska KA, Guan Z, Sessa WC. NgBR is essential for endothelial cell glycosylation and vascular development. EMBO Rep. 2016 Feb;17(2):167–177.
Journal cover image

Published In

EMBO Rep

DOI

EISSN

1469-3178

Publication Date

February 2016

Volume

17

Issue

2

Start / End Page

167 / 177

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Receptors, Cell Surface
  • Protein Processing, Post-Translational
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Mice
  • Glycosylation
  • Endothelium, Vascular
  • Developmental Biology
  • Cells, Cultured
  • Cell Proliferation