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Yin Yang 1 Promotes Thymocyte Survival by Downregulating p53.

Publication ,  Journal Article
Chen, L; Foreman, DP; Sant'Angelo, DB; Krangel, MS
Published in: J Immunol
March 15, 2016

Yin Yang 1 (YY1) is a zinc finger protein that functions as a transcriptional activator or repressor and participates in multiple biological processes, including development and tumorigenesis. To investigate the role of YY1 in developing T cells, we used mouse models that depleted YY1 at two distinct stages of thymocyte development. When YY1 was depleted in CD4(-)CD8(-) double-negative thymocytes, development to the CD4(+)CD8(+) double-positive stage was impaired, due to increased apoptosis that prevented expansion of post-β-selection thymocytes. When YY1 was depleted in double-positive thymocytes, they underwent increased cell-autonomous apoptosis in vitro and displayed a shorter lifespan in vivo, as judged by their ability to undergo secondary Vα-to-Jα recombination. Mechanistically, we found that the increased apoptosis in YY1-deficient thymocytes was attributed to overexpression of p53, because concurrent loss of p53 completely rescued the developmental defects of YY1-deficient thymocytes. These results indicated that YY1 functions as a critical regulator of thymocyte survival and that it does so by suppressing the expression of p53.

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Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

March 15, 2016

Volume

196

Issue

6

Start / End Page

2572 / 2582

Location

United States

Related Subject Headings

  • YY1 Transcription Factor
  • Tumor Suppressor Protein p53
  • Transcription, Genetic
  • Thymocytes
  • Real-Time Polymerase Chain Reaction
  • Mice, Transgenic
  • Mice, Knockout
  • Mice
  • Lymphopoiesis
  • Immunology
 

Citation

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Chen, L., Foreman, D. P., Sant’Angelo, D. B., & Krangel, M. S. (2016). Yin Yang 1 Promotes Thymocyte Survival by Downregulating p53. J Immunol, 196(6), 2572–2582. https://doi.org/10.4049/jimmunol.1501916
Chen, Liang, Daniel P. Foreman, Derek B. Sant’Angelo, and Michael S. Krangel. “Yin Yang 1 Promotes Thymocyte Survival by Downregulating p53.J Immunol 196, no. 6 (March 15, 2016): 2572–82. https://doi.org/10.4049/jimmunol.1501916.
Chen L, Foreman DP, Sant’Angelo DB, Krangel MS. Yin Yang 1 Promotes Thymocyte Survival by Downregulating p53. J Immunol. 2016 Mar 15;196(6):2572–82.
Chen, Liang, et al. “Yin Yang 1 Promotes Thymocyte Survival by Downregulating p53.J Immunol, vol. 196, no. 6, Mar. 2016, pp. 2572–82. Pubmed, doi:10.4049/jimmunol.1501916.
Chen L, Foreman DP, Sant’Angelo DB, Krangel MS. Yin Yang 1 Promotes Thymocyte Survival by Downregulating p53. J Immunol. 2016 Mar 15;196(6):2572–2582.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

March 15, 2016

Volume

196

Issue

6

Start / End Page

2572 / 2582

Location

United States

Related Subject Headings

  • YY1 Transcription Factor
  • Tumor Suppressor Protein p53
  • Transcription, Genetic
  • Thymocytes
  • Real-Time Polymerase Chain Reaction
  • Mice, Transgenic
  • Mice, Knockout
  • Mice
  • Lymphopoiesis
  • Immunology