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Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics.

Publication ,  Journal Article
Ngo, HT; Gandra, N; Fales, AM; Taylor, SM; Vo-Dinh, T
Published in: Biosens Bioelectron
July 15, 2016

One of the major obstacles to implement nucleic acid-based molecular diagnostics at the point-of-care (POC) and in resource-limited settings is the lack of sensitive and practical DNA detection methods that can be seamlessly integrated into portable platforms. Herein we present a sensitive yet simple DNA detection method using a surface-enhanced Raman scattering (SERS) nanoplatform: the ultrabright SERS nanorattle. The method, referred to as the nanorattle-based method, involves sandwich hybridization of magnetic beads that are loaded with capture probes, target sequences, and ultrabright SERS nanorattles that are loaded with reporter probes. Upon hybridization, a magnet was applied to concentrate the hybridization sandwiches at a detection spot for SERS measurements. The ultrabright SERS nanorattles, composed of a core and a shell with resonance Raman reporters loaded in the gap space between the core and the shell, serve as SERS tags for signal detection. Using this method, a specific DNA sequence of the malaria parasite Plasmodium falciparum could be detected with a detection limit of approximately 100 attomoles. Single nucleotide polymorphism (SNP) discrimination of wild type malaria DNA and mutant malaria DNA, which confers resistance to artemisinin drugs, was also demonstrated. These test models demonstrate the molecular diagnostic potential of the nanorattle-based method to both detect and genotype infectious pathogens. Furthermore, the method's simplicity makes it a suitable candidate for integration into portable platforms for POC and in resource-limited settings applications.

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Published In

Biosens Bioelectron

DOI

EISSN

1873-4235

Publication Date

July 15, 2016

Volume

81

Start / End Page

8 / 14

Location

England

Related Subject Headings

  • Spectrum Analysis, Raman
  • Sensitivity and Specificity
  • Polymorphism, Single Nucleotide
  • Point-of-Care Systems
  • Plasmodium falciparum
  • Nucleic Acid Hybridization
  • Nanostructures
  • Malaria
  • Magnets
  • Magnetic Fields
 

Citation

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Ngo, H. T., Gandra, N., Fales, A. M., Taylor, S. M., & Vo-Dinh, T. (2016). Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics. Biosens Bioelectron, 81, 8–14. https://doi.org/10.1016/j.bios.2016.01.073
Ngo, Hoan T., Naveen Gandra, Andrew M. Fales, Steve M. Taylor, and Tuan Vo-Dinh. “Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics.Biosens Bioelectron 81 (July 15, 2016): 8–14. https://doi.org/10.1016/j.bios.2016.01.073.
Ngo HT, Gandra N, Fales AM, Taylor SM, Vo-Dinh T. Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics. Biosens Bioelectron. 2016 Jul 15;81:8–14.
Ngo, Hoan T., et al. “Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics.Biosens Bioelectron, vol. 81, July 2016, pp. 8–14. Pubmed, doi:10.1016/j.bios.2016.01.073.
Ngo HT, Gandra N, Fales AM, Taylor SM, Vo-Dinh T. Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics. Biosens Bioelectron. 2016 Jul 15;81:8–14.
Journal cover image

Published In

Biosens Bioelectron

DOI

EISSN

1873-4235

Publication Date

July 15, 2016

Volume

81

Start / End Page

8 / 14

Location

England

Related Subject Headings

  • Spectrum Analysis, Raman
  • Sensitivity and Specificity
  • Polymorphism, Single Nucleotide
  • Point-of-Care Systems
  • Plasmodium falciparum
  • Nucleic Acid Hybridization
  • Nanostructures
  • Malaria
  • Magnets
  • Magnetic Fields