Skip to main content
Journal cover image

Lipid profile of human synovial fluid following intra-articular ankle fracture.

Publication ,  Journal Article
Leimer, EM; Pappan, KL; Nettles, DL; Bell, RD; Easley, ME; Olson, SA; Setton, LA; Adams, SB
Published in: J Orthop Res
March 2017

This study characterizes the metabolic profile of synovial fluid after intra-articular ankle fracture with an emphasis on changes in the lipid profile. Bilateral ankle synovial fluid from 19 patients with unilateral intra-articular ankle fracture was submitted for metabolic profiling. Contralateral ankle synovial fluid from each patient served as a matched control. Seven patients participated in a second bilateral synovial fluid collection after 6 months. Random forest classification, matched pairs t-tests (α < 0.01), repeated measures ANOVA with post-test contrasts (α < 0.01), correlation to cytokines and matrix metalloproteinases, and fracture and injury classification analyses yielded key lipid biomarkers in synovial fluid following intra-articular fracture. Free fatty acids, sphingomyelins, and lysolipids demonstrated significant elevation in fractured ankles at baseline. Fatty acids and sphingomyelins showed a significant decrease 6 months post-surgery. Random forest analysis showed predominantly fatty acids differentiating between groups. Significant correlations included fatty acids, sphingomyelins, and lysolipids with inflammatory cytokines and matrix metalloproteinases. Fracture classification showed increased fatty acids, lysolipids, and inositol metabolites as fracture severity increased. Fatty acid and sn-1 lysolipid elevation could be detrimental to the joint, as these strongly correlated with matrix metalloproteinases and TNF-α. This elevation also suggests involvement of phospholipase A2 , a potential target for therapeutic intervention. Together with elevated 2-hydroxyl fatty acids, these findings suggest elevated sn-1 lysolipids, sphingomyelins, and subsequent lipid metabolites in synovial fluid as biomarkers of ankle injury. Reversal of this signature after 6 months suggests temporary involvement of these metabolites in disease progression, although they may activate signaling pathways which drive progression to osteoarthritis. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:657-666, 2017.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

March 2017

Volume

35

Issue

3

Start / End Page

657 / 666

Location

United States

Related Subject Headings

  • Young Adult
  • Synovial Fluid
  • Retrospective Studies
  • Orthopedics
  • Middle Aged
  • Male
  • Lipid Metabolism
  • Humans
  • Female
  • Ankle Fractures
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Leimer, E. M., Pappan, K. L., Nettles, D. L., Bell, R. D., Easley, M. E., Olson, S. A., … Adams, S. B. (2017). Lipid profile of human synovial fluid following intra-articular ankle fracture. J Orthop Res, 35(3), 657–666. https://doi.org/10.1002/jor.23217
Leimer, Elizabeth M., Kirk L. Pappan, Dana L. Nettles, Richard D. Bell, Mark E. Easley, Steven A. Olson, Lori A. Setton, and Samuel B. Adams. “Lipid profile of human synovial fluid following intra-articular ankle fracture.J Orthop Res 35, no. 3 (March 2017): 657–66. https://doi.org/10.1002/jor.23217.
Leimer EM, Pappan KL, Nettles DL, Bell RD, Easley ME, Olson SA, et al. Lipid profile of human synovial fluid following intra-articular ankle fracture. J Orthop Res. 2017 Mar;35(3):657–66.
Leimer, Elizabeth M., et al. “Lipid profile of human synovial fluid following intra-articular ankle fracture.J Orthop Res, vol. 35, no. 3, Mar. 2017, pp. 657–66. Pubmed, doi:10.1002/jor.23217.
Leimer EM, Pappan KL, Nettles DL, Bell RD, Easley ME, Olson SA, Setton LA, Adams SB. Lipid profile of human synovial fluid following intra-articular ankle fracture. J Orthop Res. 2017 Mar;35(3):657–666.
Journal cover image

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

March 2017

Volume

35

Issue

3

Start / End Page

657 / 666

Location

United States

Related Subject Headings

  • Young Adult
  • Synovial Fluid
  • Retrospective Studies
  • Orthopedics
  • Middle Aged
  • Male
  • Lipid Metabolism
  • Humans
  • Female
  • Ankle Fractures