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Reduced DNA double-strand break repair capacity and risk of squamous cell carcinoma of the head and neck--A case-control study.

Publication ,  Journal Article
Liu, Z; Liu, H; Gao, F; Dahlstrom, KR; Sturgis, EM; Wei, Q
Published in: DNA Repair (Amst)
April 2016

Tobacco smoke and alcohol use play important roles in the etiology of squamous cell carcinoma of the head and neck (SCCHN). Smoking causes DNA damage, including double-strand DNA breaks (DSBs), that leads to carcinogenesis. To test the hypothesis that suboptimal DSB repair capacity is associated with risk of SCCHN, we applied a flow cytometry-based method to detect the DSB repair phenotype first in four EBV-immortalized human lymphoblastoid cell lines and then in human peripheral blood T-lymphocytes (PBTLs). With this blood-based laboratory assay, we conducted a pilot case-control study of 100 patients with newly diagnosed, previously untreated SCCHN and 124 cancer-free controls of non-Hispanic whites. We found that the mean DSB repair capacity level was significantly lower in cases (42.1%) than that in controls (54.4%) (P<0.001). When we used the median DSB repair capacity level in the controls as the cutoff value for calculating the odds ratios (ORs) with adjustment for age, sex, smoking and drinking status, the cases were more likely than the controls to have a reduced DSB repair capacity (adjusted OR=1.93; 95% confidence interval, CI=1.04-3.56, P=0.037), especially for those subjects who were ever drinkers (adjusted OR=2.73; 95% CI=1.17-6.35, P=0.020) and had oropharyngeal tumors (adjusted OR=2.17; 95% CI=1.06-4.45, P=0.035). In conclusion, these findings suggest that individuals with a reduced DSB repair capacity may be at an increased risk of developing SCCHN. Larger studies are warranted to confirm these preliminary findings.

Duke Scholars

Published In

DNA Repair (Amst)

DOI

EISSN

1568-7856

Publication Date

April 2016

Volume

40

Start / End Page

18 / 26

Location

Netherlands

Related Subject Headings

  • Smoking
  • Recombinational DNA Repair
  • Middle Aged
  • Male
  • Humans
  • Head and Neck Neoplasms
  • Female
  • Developmental Biology
  • DNA Breaks, Double-Stranded
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
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Liu, Z., Liu, H., Gao, F., Dahlstrom, K. R., Sturgis, E. M., & Wei, Q. (2016). Reduced DNA double-strand break repair capacity and risk of squamous cell carcinoma of the head and neck--A case-control study. DNA Repair (Amst), 40, 18–26. https://doi.org/10.1016/j.dnarep.2016.02.003
Liu, Zhensheng, Hongliang Liu, Fengqin Gao, Kristina R. Dahlstrom, Erich M. Sturgis, and Qingyi Wei. “Reduced DNA double-strand break repair capacity and risk of squamous cell carcinoma of the head and neck--A case-control study.DNA Repair (Amst) 40 (April 2016): 18–26. https://doi.org/10.1016/j.dnarep.2016.02.003.
Liu Z, Liu H, Gao F, Dahlstrom KR, Sturgis EM, Wei Q. Reduced DNA double-strand break repair capacity and risk of squamous cell carcinoma of the head and neck--A case-control study. DNA Repair (Amst). 2016 Apr;40:18–26.
Liu, Zhensheng, et al. “Reduced DNA double-strand break repair capacity and risk of squamous cell carcinoma of the head and neck--A case-control study.DNA Repair (Amst), vol. 40, Apr. 2016, pp. 18–26. Pubmed, doi:10.1016/j.dnarep.2016.02.003.
Liu Z, Liu H, Gao F, Dahlstrom KR, Sturgis EM, Wei Q. Reduced DNA double-strand break repair capacity and risk of squamous cell carcinoma of the head and neck--A case-control study. DNA Repair (Amst). 2016 Apr;40:18–26.
Journal cover image

Published In

DNA Repair (Amst)

DOI

EISSN

1568-7856

Publication Date

April 2016

Volume

40

Start / End Page

18 / 26

Location

Netherlands

Related Subject Headings

  • Smoking
  • Recombinational DNA Repair
  • Middle Aged
  • Male
  • Humans
  • Head and Neck Neoplasms
  • Female
  • Developmental Biology
  • DNA Breaks, Double-Stranded
  • Cells, Cultured