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Colonoscopic injection for murine solid cecal cancer model.

Publication ,  Journal Article
Karas, JR; Essani, R; Haughn, C; Uchal, M; Bishawi, MM; Bergamaschi, R
Published in: Surgical endoscopy
September 2011

The validity of current animal colon cancer models is questionable. This study was performed to evaluate whether colonoscopic injection of a murine colon cancer cell line into the cecal wall of immunocompetent rats leads to a solid tumor.A bolus of bowel prep was given to BD-IX rats. Anesthesia was injected intraperitoneally. Video fiberscope allowed for irrigation and suction. Failure was inability to reach/inject cecum. Procedure was performed by four surgeons; 100 μl of colon tumor cell suspension (DHD/K12TRb; 10 million cells in 0.1 ml) was injected into cecal wall with 23-gauge needle placed on 3 mm wire resulting in a blister. Rats were allowed to recover. Solid tumor growth was measured at scheduled necropsy at 4 weeks. Sample size (107 rats: type I error 0.05; power 80%) was based on a pilot study. Data were presented as median (range).A total of 107 male BD-IX retired breeders weighing 356 g (range 256-432 g) underwent colonoscopy with submucosal injection of cecal wall. A single solid cecal cancer was identified in 98 (91.5%) rats at scheduled necropsy. Histology confirmed adenocarcinoma with tumor size of 4 mm (range 2.6-8.4 mm). Peritoneal carcinomatosis was found in ten (9.3%) rats. Distant metastases were found in three (2.8%) rats. Complications occurred in four (3.8%) rats: two aspirations and two colon perforations.A solid cecal tumor without carcinomatosis or metastasis has been developed by colonoscopic injection of a rat colon cancer cell line in 79% of immunocompetent rats.

Duke Scholars

Published In

Surgical endoscopy

DOI

EISSN

1432-2218

ISSN

0930-2794

Publication Date

September 2011

Volume

25

Issue

9

Start / End Page

2956 / 2959

Related Subject Headings

  • Transplantation, Heterologous
  • Surgery
  • Rats
  • Prospective Studies
  • Neoplasm Transplantation
  • Mice
  • Male
  • Injections
  • Feasibility Studies
  • Colonoscopy
 

Citation

APA
Chicago
ICMJE
MLA
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Karas, J. R., Essani, R., Haughn, C., Uchal, M., Bishawi, M. M., & Bergamaschi, R. (2011). Colonoscopic injection for murine solid cecal cancer model. Surgical Endoscopy, 25(9), 2956–2959. https://doi.org/10.1007/s00464-011-1651-z
Karas, Joshua R., Rahila Essani, Christopher Haughn, Miroslav Uchal, Muath M. Bishawi, and Roberto Bergamaschi. “Colonoscopic injection for murine solid cecal cancer model.Surgical Endoscopy 25, no. 9 (September 2011): 2956–59. https://doi.org/10.1007/s00464-011-1651-z.
Karas JR, Essani R, Haughn C, Uchal M, Bishawi MM, Bergamaschi R. Colonoscopic injection for murine solid cecal cancer model. Surgical endoscopy. 2011 Sep;25(9):2956–9.
Karas, Joshua R., et al. “Colonoscopic injection for murine solid cecal cancer model.Surgical Endoscopy, vol. 25, no. 9, Sept. 2011, pp. 2956–59. Epmc, doi:10.1007/s00464-011-1651-z.
Karas JR, Essani R, Haughn C, Uchal M, Bishawi MM, Bergamaschi R. Colonoscopic injection for murine solid cecal cancer model. Surgical endoscopy. 2011 Sep;25(9):2956–2959.
Journal cover image

Published In

Surgical endoscopy

DOI

EISSN

1432-2218

ISSN

0930-2794

Publication Date

September 2011

Volume

25

Issue

9

Start / End Page

2956 / 2959

Related Subject Headings

  • Transplantation, Heterologous
  • Surgery
  • Rats
  • Prospective Studies
  • Neoplasm Transplantation
  • Mice
  • Male
  • Injections
  • Feasibility Studies
  • Colonoscopy