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Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells

Publication ,  Journal Article
Zhang, HM; Zhang, LW; Ren, J; Fan, L; Si, XM; Liu, WC
Published in: Cellular Immunology
2006

alpha-Fetoprotein (AFP) may be a possible target for a hepatocellular carcinoma (HCC)-specific vaccination. But some studies have demonstrated that dendritic cells (DCs) treated with AFP become dysfunctional. So in this study, we try to transfect AFP mRNA into DCs and observe the ability of DCs to induce AFP-specific CD4(+) and CD8(+) T cells. We hope that AFP can be processed and presented by DCs directly, rather than released to the cultures. So there will be no AFP negative effect on the function of DCs. In the study, immature DCs generated from peripheral blood mononuclear cells (PBMCs) of HLA-A2(+) HCC patients were transfected with AFP mRNA. Then the transfected, matured DCs were used to stimulate autologous T cells. The results showed that the expressions of membrane molecules of DCs after transfection were increased dramatically, and interleukin-12 (IL-12) p70 release in the supernatant was elevated significantly. There was only a minority of AFP release in the supernatants of transfected DCs. CTLs induced by the transfected DCs recognized HLA-matched AFP positive HepG2 cell line specifically and the AFP-specific proliferative T-cell responses could also be induced. These findings indicate that this AFP mRNA transfection strategy could generate fully functional DCs, which could induce specific T cells to recognize AFP(+) HCC cells. (c) 2006 Elsevier Inc. All rights reserved.

Duke Scholars

Published In

Cellular Immunology

DOI

ISSN

0008-8749

Publication Date

2006

Volume

239

Start / End Page

144 / 150

Related Subject Headings

  • Immunology
  • 1107 Immunology
 

Citation

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Zhang, H. M., Zhang, L. W., Ren, J., Fan, L., Si, X. M., & Liu, W. C. (2006). Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells. Cellular Immunology, 239, 144–150. https://doi.org/10.1016/j.cellimm.2006.05.004
Zhang, H. M., L. W. Zhang, J. Ren, L. Fan, X. M. Si, and W. C. Liu. “Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells.” Cellular Immunology 239 (2006): 144–50. https://doi.org/10.1016/j.cellimm.2006.05.004.
Zhang HM, Zhang LW, Ren J, Fan L, Si XM, Liu WC. Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells. Cellular Immunology. 2006;239:144–50.
Zhang, H. M., et al. “Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells.” Cellular Immunology, vol. 239, 2006, pp. 144–50. Manual, doi:10.1016/j.cellimm.2006.05.004.
Zhang HM, Zhang LW, Ren J, Fan L, Si XM, Liu WC. Induction of alpha-fetoprotein-specific CD4-and CD8-mediated T-cell response using RNA-transfected dendritic cells. Cellular Immunology. 2006;239:144–150.
Journal cover image

Published In

Cellular Immunology

DOI

ISSN

0008-8749

Publication Date

2006

Volume

239

Start / End Page

144 / 150

Related Subject Headings

  • Immunology
  • 1107 Immunology