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Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits.

Publication ,  Journal Article
Lund, DD; Faraci, FM; Miller, FJ; Heistad, DD
Published in: Circulation
March 7, 2000

BACKGROUND: Diabetes mellitus is associated with impairment of NO-mediated vascular relaxation. The purpose of this study was to determine whether adenovirus-mediated gene transfer of endothelial NO synthase (eNOS) or Cu/Zn superoxide dismutase (SOD1) improves responsiveness to acetylcholine in alloxan-induced diabetic rabbits. METHODS AND RESULTS: After 8 weeks, plasma glucose was greater in diabetic rabbits (418+/-35 mg/dL) (mean+/-SEM) than in normal rabbits (105+/-4 mg/dL). Carotid arteries were removed and cut into ring segments. Arteries were incubated for 2 hours with adenoviral vectors driven by a CMV promoter expressing beta-galactosidase (beta-gal), eNOS, SOD1, or vehicle. After incubation with virus, arteries were incubated for an additional 24 hours to allow transgene expression. Vascular reactivity was examined by recording isometric tension. After precontraction with phenylephrine, responses to the endothelium-independent vasodilator sodium nitroprusside were similar in diabetic and normal arteries. Endothelium-dependent relaxation to acetylcholine (3x10(-6) mol/L) was significantly less in arteries from diabetic animals (68+/-5%) than in normal vessels (90+/-3%). Adenoviral transfection of arteries with eNOS improved relaxation in response to acetylcholine in diabetic (EC(50) eNOS=0.64+/-0.12x10(-7) mol/L versus vehicle =1. 70+/-0.43x10(-7) mol/L) but not normal arteries. Vasorelaxation in response to acetylcholine was inhibited by N(omega)-nitro-L-arginine (100 micromol/L) in all groups. Responses to acetylcholine were unchanged after gene transfection of SOD1 or beta-gal in arteries from diabetic or normal rabbits. CONCLUSIONS: Adenovirus-mediated gene transfer of eNOS, but not SOD, improves impaired NO-mediated relaxation in vessels from diabetic rabbits.

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Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

March 7, 2000

Volume

101

Issue

9

Start / End Page

1027 / 1033

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Vasomotor System
  • Vasodilator Agents
  • Vasodilation
  • Superoxides
  • Rabbits
  • Nitroarginine
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase
  • Male
 

Citation

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Lund, D. D., Faraci, F. M., Miller, F. J., & Heistad, D. D. (2000). Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits. Circulation, 101(9), 1027–1033. https://doi.org/10.1161/01.cir.101.9.1027
Lund, D. D., F. M. Faraci, F. J. Miller, and D. D. Heistad. “Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits.Circulation 101, no. 9 (March 7, 2000): 1027–33. https://doi.org/10.1161/01.cir.101.9.1027.
Lund DD, Faraci FM, Miller FJ, Heistad DD. Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits. Circulation. 2000 Mar 7;101(9):1027–33.
Lund, D. D., et al. “Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits.Circulation, vol. 101, no. 9, Mar. 2000, pp. 1027–33. Pubmed, doi:10.1161/01.cir.101.9.1027.
Lund DD, Faraci FM, Miller FJ, Heistad DD. Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits. Circulation. 2000 Mar 7;101(9):1027–1033.

Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

March 7, 2000

Volume

101

Issue

9

Start / End Page

1027 / 1033

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Vasomotor System
  • Vasodilator Agents
  • Vasodilation
  • Superoxides
  • Rabbits
  • Nitroarginine
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase
  • Male