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Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth.

Publication ,  Journal Article
Golub, JS; Kim, Y-T; Duvall, CL; Bellamkonda, RV; Gupta, D; Lin, AS; Weiss, D; Robert Taylor, W; Guldberg, RE
Published in: American journal of physiology. Heart and circulatory physiology
June 2010

Technologies to increase tissue vascularity are critically important to the fields of tissue engineering and cardiovascular medicine. Currently, limited technologies exist to encourage angiogenesis and arteriogenesis in a controlled manner. In the present study, we describe an injectable controlled release system consisting of VEGF encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). The majority of VEGF was released gradually over 2-4 days from the NPs as determined by an ELISA release kinetics experiment. An in vitro aortic ring bioassay was used to verify the bioactivity of VEGF-NPs compared with empty NPs and no treatment. A mouse femoral artery ischemia model was then used to measure revascularization in VEGF-NP-treated limbs compared with limbs treated with naked VEGF and saline. 129/Sv mice were anesthetized with isoflurane, and a region of the common femoral artery and vein was ligated and excised. Mice were then injected with VEGF-NPs, naked VEGF, or saline. After 4 days, three-dimensional microcomputed tomography angiography was used to quantify vessel growth and morphology. Mice that received VEGF-NP treatment showed a significant increase in total vessel volume and vessel connectivity compared with 5 microg VEGF, 2.5 microg VEGF, and saline treatment (all P < 0.001). When the yield of the fabrication process was taken into account, VEGF-NPs were over an order of magnitude more potent than naked VEGF in increasing blood vessel volume. Differences between the VEGF-NP group and all other groups were even greater when only small-sized vessels under 300 mum diameter were analyzed. In conclusion, sustained VEGF delivery via PLGA NPs shows promise for encouraging blood vessel growth in tissue engineering and cardiovascular medicine applications.

Duke Scholars

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

June 2010

Volume

298

Issue

6

Start / End Page

H1959 / H1965

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tomography, X-Ray Computed
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Neovascularization, Physiologic
  • Nanoparticles
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lactic Acid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Golub, J. S., Kim, Y.-T., Duvall, C. L., Bellamkonda, R. V., Gupta, D., Lin, A. S., … Guldberg, R. E. (2010). Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth. American Journal of Physiology. Heart and Circulatory Physiology, 298(6), H1959–H1965. https://doi.org/10.1152/ajpheart.00199.2009
Golub, Justin S., Young-tae Kim, Craig L. Duvall, Ravi V. Bellamkonda, Divya Gupta, Angela S. Lin, Daiana Weiss, W. Robert Taylor, and Robert E. Guldberg. “Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth.American Journal of Physiology. Heart and Circulatory Physiology 298, no. 6 (June 2010): H1959–65. https://doi.org/10.1152/ajpheart.00199.2009.
Golub JS, Kim Y-T, Duvall CL, Bellamkonda RV, Gupta D, Lin AS, et al. Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth. American journal of physiology Heart and circulatory physiology. 2010 Jun;298(6):H1959–65.
Golub, Justin S., et al. “Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth.American Journal of Physiology. Heart and Circulatory Physiology, vol. 298, no. 6, June 2010, pp. H1959–65. Epmc, doi:10.1152/ajpheart.00199.2009.
Golub JS, Kim Y-T, Duvall CL, Bellamkonda RV, Gupta D, Lin AS, Weiss D, Robert Taylor W, Guldberg RE. Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth. American journal of physiology Heart and circulatory physiology. 2010 Jun;298(6):H1959–H1965.

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

June 2010

Volume

298

Issue

6

Start / End Page

H1959 / H1965

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tomography, X-Ray Computed
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Neovascularization, Physiologic
  • Nanoparticles
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lactic Acid