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S-Nitrosylated fetal hemoglobin in neonatal human blood.

Publication ,  Journal Article
Riccio, DA; Malowitz, JR; Cotten, CM; Murtha, AP; McMahon, TJ
Published in: Biochem Biophys Res Commun
May 13, 2016

BACKGROUND: Nitric oxide (NO) and its derivatives play important roles in the cardiopulmonary transition upon birth and in other oxygen-sensitive developmental milestones. One mechanism for the coupling of oxygen sensing and signaling by NO species is via the formation of an S-nitrosothiol (SNO) moiety on hemoglobin (Hb, forming SNO-Hb) and its release from the red blood cell in hypoxia. Although SNO-Hb formed on adult-type Hb (HbA, forming SNO-HbA) has been documented in physiological and pathophysiological human states, the fetal variant, SNO-HbF, has thus far not been isolated or characterized in human blood. METHODS AND RESULTS: We developed a technique capable of separating Hbs A and F under conditions that preserve SNO. We then measured SNO-HbF in the blood of healthy and premature or otherwise ill neonates using the gold standard for SNO measurement, mercury-coupled photolysis-chemiluminescence. SNO-HbF levels were in the range of those previously reported for HbA in adults. We found that SNO-HbF was more abundant at earlier gestational age (<30 weeks), even when accounting for the absolute HbF level. CONCLUSIONS: The ability to monitor SNO-HbF could provide new insights into fetal development and the perinatal transition, and has potential as a biomarker relevant to the management of neonatal diseases.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

May 13, 2016

Volume

473

Issue

4

Start / End Page

1084 / 1089

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Reproducibility of Results
  • Male
  • Infant, Newborn
  • Humans
  • Hemoglobins
  • Gestational Age
  • Fetal Blood
  • Female
  • Chromatography, Ion Exchange
 

Citation

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Riccio, D. A., Malowitz, J. R., Cotten, C. M., Murtha, A. P., & McMahon, T. J. (2016). S-Nitrosylated fetal hemoglobin in neonatal human blood. Biochem Biophys Res Commun, 473(4), 1084–1089. https://doi.org/10.1016/j.bbrc.2016.04.019
Riccio, Daniel A., Jonathan R. Malowitz, C Michael Cotten, Amy P. Murtha, and Timothy J. McMahon. “S-Nitrosylated fetal hemoglobin in neonatal human blood.Biochem Biophys Res Commun 473, no. 4 (May 13, 2016): 1084–89. https://doi.org/10.1016/j.bbrc.2016.04.019.
Riccio DA, Malowitz JR, Cotten CM, Murtha AP, McMahon TJ. S-Nitrosylated fetal hemoglobin in neonatal human blood. Biochem Biophys Res Commun. 2016 May 13;473(4):1084–9.
Riccio, Daniel A., et al. “S-Nitrosylated fetal hemoglobin in neonatal human blood.Biochem Biophys Res Commun, vol. 473, no. 4, May 2016, pp. 1084–89. Pubmed, doi:10.1016/j.bbrc.2016.04.019.
Riccio DA, Malowitz JR, Cotten CM, Murtha AP, McMahon TJ. S-Nitrosylated fetal hemoglobin in neonatal human blood. Biochem Biophys Res Commun. 2016 May 13;473(4):1084–1089.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

May 13, 2016

Volume

473

Issue

4

Start / End Page

1084 / 1089

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Reproducibility of Results
  • Male
  • Infant, Newborn
  • Humans
  • Hemoglobins
  • Gestational Age
  • Fetal Blood
  • Female
  • Chromatography, Ion Exchange