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Impact of intestinal electrical stimulation on nutrient-induced GLP-1 secretion in vivo.

Publication ,  Journal Article
Sandoval, D; Dunki-Jacobs, A; Sorrell, J; Seeley, RJ; D'Alessio, DD
Published in: Neurogastroenterol Motil
August 2013

BACKGROUND: Increases in L-cell release of GLP-1 are proposed to serve as a negative feedback signal for postprandial changes in gastric emptying and/or motility. Previous ex vivo data suggests that direct electrical stimulation (E-stim) of ileal segments stimulates secretion of GLP-1. This suggests potential feed-forward increases in GLP-1 driven by intestinal neuronal and/or motor activity. METHODS: To determine if E-stim could increase GLP-1 levels in an in vivo setting, we administered E-stim and nutrients to male Long- Evans rats (300-350 g) under general anesthesia. KEY RESULTS: Nutrient infusion into the duodenum or ileum significantly increased plasma GLP-1 levels, but E-stim applied to these locations did not (P < 0.05). However, the combination of E-stim and nutrient infusion, in either the ileum or duodenum, significantly increased plasma GLP-1 when compared to nutrient infusion alone (P < 0.05), and this effect was not blocked by either norepinephrine or atropine. To test the impact of intestinal motor activity, the effect of extra-luminal mechanical stimulation (M-stim) on GLP-1 levels was assessed. In the duodenum, but not the ileum, M-stim plus nutrient infusion significantly increased GLP-1 over nutrient infusion or M-stim alone (P < 0.05). CONCLUSIONS & INFERENCES: Thus, both E- and M-stim of the duodenum, but only E-stim of the ileum augmented nutrient-stimulated GLP-1 release. These data demonstrate that factors beyond enteral nutrients could contribute to the regulation of GLP-1 secretion.

Duke Scholars

Published In

Neurogastroenterol Motil

DOI

EISSN

1365-2982

Publication Date

August 2013

Volume

25

Issue

8

Start / End Page

700 / 705

Location

England

Related Subject Headings

  • Rats, Long-Evans
  • Rats
  • Male
  • Ileum
  • Glucagon-Like Peptide 1
  • Gastroenterology & Hepatology
  • Food
  • Electric Stimulation
  • Duodenum
  • Animals
 

Citation

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Sandoval, D., Dunki-Jacobs, A., Sorrell, J., Seeley, R. J., & D’Alessio, D. D. (2013). Impact of intestinal electrical stimulation on nutrient-induced GLP-1 secretion in vivo. Neurogastroenterol Motil, 25(8), 700–705. https://doi.org/10.1111/nmo.12152
Sandoval, D., A. Dunki-Jacobs, J. Sorrell, R. J. Seeley, and D. D. D’Alessio. “Impact of intestinal electrical stimulation on nutrient-induced GLP-1 secretion in vivo.Neurogastroenterol Motil 25, no. 8 (August 2013): 700–705. https://doi.org/10.1111/nmo.12152.
Sandoval D, Dunki-Jacobs A, Sorrell J, Seeley RJ, D’Alessio DD. Impact of intestinal electrical stimulation on nutrient-induced GLP-1 secretion in vivo. Neurogastroenterol Motil. 2013 Aug;25(8):700–5.
Sandoval, D., et al. “Impact of intestinal electrical stimulation on nutrient-induced GLP-1 secretion in vivo.Neurogastroenterol Motil, vol. 25, no. 8, Aug. 2013, pp. 700–05. Pubmed, doi:10.1111/nmo.12152.
Sandoval D, Dunki-Jacobs A, Sorrell J, Seeley RJ, D’Alessio DD. Impact of intestinal electrical stimulation on nutrient-induced GLP-1 secretion in vivo. Neurogastroenterol Motil. 2013 Aug;25(8):700–705.
Journal cover image

Published In

Neurogastroenterol Motil

DOI

EISSN

1365-2982

Publication Date

August 2013

Volume

25

Issue

8

Start / End Page

700 / 705

Location

England

Related Subject Headings

  • Rats, Long-Evans
  • Rats
  • Male
  • Ileum
  • Glucagon-Like Peptide 1
  • Gastroenterology & Hepatology
  • Food
  • Electric Stimulation
  • Duodenum
  • Animals