Apolipoprotein A-IV interacts synergistically with melanocortins to reduce food intake.
Apolipoprotein (apo) A-IV is an anorexigenic gastrointestinal peptide that is also synthesized in the hypothalamus. The goal of these experiments was to determine whether apo A-IV interacts with the central melanocortin (MC) system in the control of feeding. The third ventricular (i3vt) administration of a subthreshold dose of apo A-IV (0.5 microg) potentiated i3vt MC-induced (metallothionein-II, 0.03 nmol) suppression of 30-min feeding in Long-Evans rats. A subthreshold dose of the MC antagonist (SHU9119, 0.1 nmol, i3vt) completely attenuated the anorectic effect of i3vt apo A-IV (1.5 microg). The i3vt apo A-IV significantly elevated the expression of c-Fos in neurons of the paraventricular nucleus of the hypothalamus, but not in the arcuate nucleus or median eminence. In addition, c-Fos expression was not colocalized with proopiomelanocortin-positive neurons. These data support a synergistic interaction between apo A-IV and melanocortins that reduces food intake by acting downstream of the arcuate.
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- Rats, Long-Evans
- Rats
- Pro-Opiomelanocortin
- Physiology
- Neurons
- Metallothionein
- Melanocyte-Stimulating Hormones
- Male
- Hypothalamus
- Genes, fos
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Rats, Long-Evans
- Rats
- Pro-Opiomelanocortin
- Physiology
- Neurons
- Metallothionein
- Melanocyte-Stimulating Hormones
- Male
- Hypothalamus
- Genes, fos