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Transfecting Human Monocytes with RNA.

Publication ,  Journal Article
Dannull, J; Nair, SK
Published in: Methods Mol Biol
2016

Targeting monocytes as a delivery system for drugs or nucleic acids, and thereby harnessing their natural tissue-infiltrating capacity, has become an area of intense investigation in both basic and clinical research. Herein we describe an efficient method to deliver mRNA (messenger RNA) or siRNA (small interfering RNA) into human monocytes by electroporation. This method can be applied in the laboratory to monocytes isolated via magnetic bead-based techniques, or in a clinical setting using monocytes that were collected via counterflow centrifugation elutriation using the Elutra(®) Cell Separation System. We further demonstrate that electroporation of monocytes with RNA represents a robust and highly relevant approach to modify monocytes for cell-based therapies. Last, the procedure described can readily be adapted to monocytes from different species, hence facilitating research in animal models.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2016

Volume

1428

Start / End Page

177 / 186

Location

United States

Related Subject Headings

  • Transfection
  • RNA, Small Interfering
  • RNA, Messenger
  • RNA
  • Monocytes
  • Humans
  • Electroporation
  • Electromagnetic Phenomena
  • Developmental Biology
  • Cells, Cultured
 

Citation

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MLA
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Dannull, J., & Nair, S. K. (2016). Transfecting Human Monocytes with RNA. Methods Mol Biol, 1428, 177–186. https://doi.org/10.1007/978-1-4939-3625-0_12
Dannull, Jens, and Smita K. Nair. “Transfecting Human Monocytes with RNA.Methods Mol Biol 1428 (2016): 177–86. https://doi.org/10.1007/978-1-4939-3625-0_12.
Dannull J, Nair SK. Transfecting Human Monocytes with RNA. Methods Mol Biol. 2016;1428:177–86.
Dannull, Jens, and Smita K. Nair. “Transfecting Human Monocytes with RNA.Methods Mol Biol, vol. 1428, 2016, pp. 177–86. Pubmed, doi:10.1007/978-1-4939-3625-0_12.
Dannull J, Nair SK. Transfecting Human Monocytes with RNA. Methods Mol Biol. 2016;1428:177–186.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2016

Volume

1428

Start / End Page

177 / 186

Location

United States

Related Subject Headings

  • Transfection
  • RNA, Small Interfering
  • RNA, Messenger
  • RNA
  • Monocytes
  • Humans
  • Electroporation
  • Electromagnetic Phenomena
  • Developmental Biology
  • Cells, Cultured