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Characterization of Immobilized β-N-Acetylhexosaminidase1

Publication ,  Journal Article
Nunley, JA; Baricos, WH; Chambers, RP
Published in: Analytical Letters
January 1, 1977
Published version (DOI)

The properties of β -N -acetylhexosaminidase chemically bound to Sepharose 4B were determined and compared to those of the soluble enzyme. External diffusion effects on the kinetics of the immobilized enzyme were eliminated by assaying in a recirculation reactor with rapid flow rates. The immobilized β-N-acetylhexosaminidase exhibited a broad pH optimum quite similar to that of the soluble enzyme. Compared to the soluble enzyme the immobilized enzyme demonstrated a markedly enhanced stability at each pH and temperature investigated. Immobilization caused an increase in both the apparent Km and Ki, with. © 1977, Taylor & Francis Group, LLC. All rights reserved.

Duke Scholars

James Albert Nunley II
Author James Albert Nunley II Orthopaedic Surgery

Published In

Analytical Letters

DOI

10.1080/00032717708079394

EISSN

1532-236X

ISSN

0003-2719

Publication Date

January 1, 1977

Volume

10

Issue

6

Start / End Page

501 / 514

Related Subject Headings

  • Analytical Chemistry
  • 3401 Analytical chemistry
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry
 

Citation

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Nunley, J. A., Baricos, W. H., & Chambers, R. P. (1977). Characterization of Immobilized β-N-Acetylhexosaminidase1. Analytical Letters, 10(6), 501–514. https://doi.org/10.1080/00032717708079394
Nunley, J. A., W. H. Baricos, and R. P. Chambers. “Characterization of Immobilized β-N-Acetylhexosaminidase1.” Analytical Letters 10, no. 6 (January 1, 1977): 501–14. https://doi.org/10.1080/00032717708079394.
Nunley JA, Baricos WH, Chambers RP. Characterization of Immobilized β-N-Acetylhexosaminidase1. Analytical Letters. 1977 Jan 1;10(6):501–14.
Nunley, J. A., et al. “Characterization of Immobilized β-N-Acetylhexosaminidase1.” Analytical Letters, vol. 10, no. 6, Jan. 1977, pp. 501–14. Scopus, doi:10.1080/00032717708079394.
Nunley JA, Baricos WH, Chambers RP. Characterization of Immobilized β-N-Acetylhexosaminidase1. Analytical Letters. 1977 Jan 1;10(6):501–514.

Published In

Analytical Letters

DOI

10.1080/00032717708079394

EISSN

1532-236X

ISSN

0003-2719

Publication Date

January 1, 1977

Volume

10

Issue

6

Start / End Page

501 / 514

Related Subject Headings

  • Analytical Chemistry
  • 3401 Analytical chemistry
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry